验证数据展示
经过测试的应用
| Positive WB detected in | PC-3 cells, SKOV-3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
60029-1-Ig targets KMO in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag1109 Product name: Recombinant human KMO protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 107-407 aa of BC005297 Sequence: LSVSRENLNKDLLTAAEKYPNVKMHFNHRLLKCNPEEGMITVLGSDKVPKDVTCDLIVGCDGAYSTVRSHLMKKPRFDYSQQYIPHGYMELTIPPKNGDYAMEPNYLHIWPRNTFMMIALPNMNKSFTCTLFMPFEEFEKLLTSNDVVDFFQKYFPDAIPLIGEKLLVQDFFLLPAQPMISVKCSSFHFKSHCVLLGDAAHAIVPFFGQGMNAGFEDCLVFDELMDKFSNDLSLCLPVFSRLRIPDDHAISDLSMYNYIEKNMERFLHAIMPSTFIPLYTMVTFSRIRYHEAVQRWHWQKR 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | kynurenine 3-monooxygenase (kynurenine 3-hydroxylase) |
| 别名 | 2D2F2, EC:1.14.13.9, Kynurenine 3 hydroxylase, Kynurenine 3 monooxygenase, Kynurenine 3-hydroxylase |
| 计算分子量 | 486 aa, 56 kDa |
| 观测分子量 | 56 kDa |
| GenBank蛋白编号 | BC005297 |
| 基因名称 | KMO |
| Gene ID (NCBI) | 8564 |
| RRID | AB_2133396 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | O15229 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
KMO(Kynurenine 3-monooxygenase) is a membrane protein located on the outer membrane of mitochondria. Tissue distribution studies have revealed that, in rats, highest enzyme activity is found in kidney and liver, with brain having the least activity in comparison to peripheral organs(PMID: 9237672).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for KMO antibody 60029-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Acta Pharm Sin B The crucial function of IDO1 in pulmonary fibrosis: From the perspective of mitochondrial fusion in lung fibroblasts and targeted molecular inhibition. | ||
Neuro Oncol Molecular imaging correlates of tryptophan metabolism via the kynurenine pathway in human meningiomas. | ||
Phytomedicine Ginsenoside compound K ameliorates depressive-like behaviors by targeting kynurenine 3-monooxygenase. | ||
NPJ Sci Food Dietary advanced glycation products combined with chronic restraint stress induced anxiety-like and depression-like behaviors in male mice. | ||
Int J Biol Macromol A novel recombinant peptide, rAnguillin, induces ferroptosis in nasopharyngeal carcinoma by promoting FOXM1 degradation and disrupting the FOXM1-PABPC1L-tryptophan metabolism axis. |
