验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, THP-1 cells, mouse kidney tissue, rat kidney tissue, SMMC-7721 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
29329-1-AP targets GPX1 in WB, IHC, IF, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | Human, Mouse, Rat |
| 文献引用反应性 | human, mouse, rat, pig, chicken, bovine, sheep |
| 免疫原 |
CatNo: Ag31101 Product name: Recombinant human GPX1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 102-203 aa of BC000742 Sequence: GGGFEPNFMLFEKCEVNGAGAHPLFAFLREALPAPSDDATALMTDPKLITWSPVCRNDVAWNFEKFLVGPDGVPLRRYSRRFQTIDIEPDIEALLSQGPSCA 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | glutathione peroxidase 1 |
| 别名 | glutathione peroxidase 1, GPx 1, GPX1, GSHPx 1, GSHPX1 |
| 计算分子量 | 22 kDa |
| 观测分子量 | 22 kDa |
| GenBank蛋白编号 | BC000742 |
| 基因名称 | GPX1 |
| Gene ID (NCBI) | 2876 |
| RRID | AB_2918283 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P07203 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Gpx protein, known as selenoprotein, consists selenium in its catalytic site. Gpx1 is an abundant isoform, which involves in scavenging endogenous ROS using electron provided by reduced glutathione and ubiquitously expressed the cytoplasm and mitochondria of all cell types. (PMID: 30632027)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GPX1 antibody 29329-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell A GPX1-OSBPL8 axis mediates noncanonical in vivo ferroptosis and cancer growth suppression.
| ||
Bioact Mater Mesenchymal stromal/stem cell spheroid-derived extracellular vesicles advance the therapeutic efficacy of 3D-printed vascularized artificial liver lobules in liver failure treatment | ||
Nat Neurosci Derivation and transcriptional reprogramming of border-forming wound repair astrocytes after spinal cord injury or stroke in mice | ||
Nat Commun Plant-derived hydrogel and photosynthetic nano-units for myocardial infarction therapy | ||
Redox Biol Iron(III)-salophene catalyzes redox cycles that induce phospholipid peroxidation and deplete cancer cells of ferroptosis-protecting cofactors |
