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验证数据展示

  • WB analysis using 20851-1-AP

    WB analysis using 20851-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20851-1-AP (GRINA antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 20851-1-AP

    IHC staining of human placenta using 20851-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 20851-1-AP (GRINA antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GRINA antibody included
  • IHC staining of human placenta using 20851-1-AP

    IHC staining of human placenta using 20851-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 20851-1-AP (GRINA antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GRINA antibody included
  • IHC staining of human thyroid cancer using 20851-1-AP

    IHC staining of human thyroid cancer using 20851-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 20851-1-AP (GRINA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GRINA antibody included

GRINA Polyclonal antibody

GRINA Polyclonal Antibody for WB, IHC, ELISA
Cat No. 20851-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

Glutamate [NMDA] receptor-associated protein 1, HNRGW, LFG1, Protein lifeguard 1, Putative MAPK-activating protein PM02

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, human placenta tissue
Positive IHC detected inhuman placenta tissue, rat kidney tissue, rat brain tissue, mouse adrenal gland tissue, human thyroid cancer tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy® GRINA Ready-To-Use IHC Kit. GRINA primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20851-1-AP targets GRINA in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag14716

Product name: Recombinant human GRINA protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-170 aa of BC041788

Sequence: MSHEKSFLVSGDNYPPPNPGYPGGPQPPMPPYAQPPYPGAPYPQPPFQPSPYGQPGYPHGPSPYPQGGYPQGPYPQGGYPQGPYPQEGYPQGPYPQGGYPQGPYPQNPFPPNPYGQPQVFPGQDPDSPQHGNYQEEGPPSYYDNQDFPATNWDDKSIRQAFIRKVFLVLT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 glutamate receptor, ionotropic, N-methyl D-aspartate-associated protein 1 (glutamate binding)
别名 Glutamate [NMDA] receptor-associated protein 1, HNRGW, LFG1, Protein lifeguard 1, Putative MAPK-activating protein PM02
计算分子量 371 aa, 41 kDa
观测分子量49 kDa
GenBank蛋白编号BC041788
基因名称 GRINA
Gene ID (NCBI) 2907
RRIDAB_3742005
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ7Z429
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Glutamate Receptor Ionotropic NMDA-Associated Protein 1 (GRINA, also known as TMBIM3) is a transmembrane protein involved in calcium homeostasis and cell survival. It plays a crucial role in regulating intracellular calcium levels, which are essential for processes such as cell survival, neurotransmitter release, and apoptosis (PMID: 32124700). GRINA also involves vesicle transport and sorting, particularly in secretory cells like neurons and mammary glands (PMID: 31426446). Additionally, GRINA has been implicated in regulating aerobic glycolysis and tumor progression in gastric cancer (PMID: 30541591).

实验方案

Product Specific Protocols
IHC protocol for GRINA antibody 20851-1-APDownload protocol
WB protocol for GRINA antibody 20851-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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