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验证数据展示

  • WB analysis using 18011-1-AP

    WB analysis using 18011-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18011-1-AP (NUBP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 18011-1-AP

    WB analysis of HeLa using 18011-1-AP

    WB result of NUBP1 antibody (18011-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NUBP1 transfected HeLa cells.

  • IP experiment of Jurkat using 18011-1-AP

    IP experiment of Jurkat using 18011-1-AP

    IP result of anti-NUBP1 (IP:18011-1-AP, 4ug; Detection:18011-1-AP 1:300) with Jurkat cells lysate 3600ug.

  • IHC staining of human stomach using 18011-1-AP

    IHC staining of human stomach using 18011-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 18011-1-AP (NUBP1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NUBP1 antibody included
  • IHC staining of mouse kidney using 18011-1-AP

    IHC staining of mouse kidney using 18011-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 18011-1-AP (NUBP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NUBP1 antibody included
  • IHC staining of human kidney using 18011-1-AP

    IHC staining of human kidney using 18011-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18011-1-AP (NUBP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NUBP1 antibody included
  • IHC staining of human placenta using 18011-1-AP

    IHC staining of human placenta using 18011-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 18011-1-AP (NUBP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NUBP1 antibody included
  • IHC staining of human renal cell carcinoma using 18011-1-AP

    IHC staining of human renal cell carcinoma using 18011-1-AP

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 18011-1-AP (NUBP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NUBP1 antibody included
  • IHC staining of mouse lung using 18011-1-AP

    IHC staining of mouse lung using 18011-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 18011-1-AP (NUBP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NUBP1 antibody included
  • IHC staining of rat kidney using 18011-1-AP

    IHC staining of rat kidney using 18011-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 18011-1-AP (NUBP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NUBP1 antibody included
  • Featured Product
  • KD/KO Validated

NUBP1 Polyclonal antibody

NUBP1 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 18011-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IP, ELISA

别名

Cytosolic Fe-S cluster assembly factor NUBP1, NBP, NBP 1, NBP1, Nbp35

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA549 cells, HeLa cells, LNCaP cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman stomach tissue, mouse kidney tissue, human kidney tissue, mouse lung tissue, rat kidney tissue, human renal cell carcinoma tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy® NUBP1 Ready-To-Use IHC Kit. NUBP1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

18011-1-AP targets NUBP1 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原

CatNo: Ag12510

Product name: Recombinant human NUBP1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-309 aa of BC100290

Sequence: MEEVPHDCPGADSAQAGRGASCQGCPNQRLCASGAGATPDTAIEEIKEKMKTVKHKILVLSGKGGVGKSTFSAHLAHGLAEDENTQIALLDIDICGPSIPKIMGLEGEQYVEDNLGVMSVGFLLSSPDDAVIWRGPKKNGMIKQFLRDVDWGEVDYLIVDTPPGTSDEHLSVVRYLATAHIDGAVIITTPQEVSLQDVRKEINFCRKVKLPIIGVVENMSGFICPKCKKESQIFPPTTGGAELMCQDLEVPLLGRVPLDPLIGKNCDKGQSFFIDAPDSPATLAYRSIIQRIQEFCNLHQSKEENLISS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 nucleotide binding protein 1 (MinD homolog, E. coli)
别名 Cytosolic Fe-S cluster assembly factor NUBP1, NBP, NBP 1, NBP1, Nbp35
计算分子量 309 aa, 33 kDa
观测分子量36-40 kDa
GenBank蛋白编号BC100290
基因名称 NUBP1
Gene ID (NCBI) 4682
RRIDAB_2935446
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP53384
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for NUBP1 antibody 18011-1-APDownload protocol
IP protocol for NUBP1 antibody 18011-1-APDownload protocol
WB protocol for NUBP1 antibody 18011-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Sci Adv

Autophagy supports mitochondrial metabolism through the regulation of iron homeostasis in pancreatic cancer

Authors - Subhadip Mukhopadhyay
View Article
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