验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, HuH-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14158-1-AP targets MIPOL1 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag5340 Product name: Recombinant human MIPOL1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-261 aa of BC035870 Sequence: MSRLQLAIEERDEAIARAKHMEMSLKVLENINPEENDMTLQELLNRINNADTGIAIQKNGAIIVDRIYKTKECKMRITAEEMSALIEERDAALSKCKRLEQELHHVKEQNQTSANNMRHLTAENNQERALKAKLLSMQQARETAVQQYKKLEEEIQTLRVYYSLHKSLSQEENLKDQFNYTLSTYEEALKNRENIVSITQQQNEELATQLQQALTERANMELQLQHAREASQVANEKVQKLERLVDVLRKKVGTGTMRTVI 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | mirror-image polydactyly 1 |
| 别名 | mirror image polydactyly 1, Mirror-image polydactyly gene 1 protein |
| 计算分子量 | 442 aa, 52 kDa |
| 观测分子量 | 52 kDa |
| GenBank蛋白编号 | BC035870 |
| 基因名称 | MIPOL1 |
| Gene ID (NCBI) | 145282 |
| RRID | AB_2141930 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8TD10 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MIPOL1 (also named CCDC193) encodes a nuclear coiled-coil protein. Germline MIPOL1 variants cause Laurin‑Sandrow syndrome characterized by mirror-image polydactyly. It acts as a candidate tumor suppressor in nasopharyngeal carcinoma. Downregulation of MIPOL1 activates AKT/FAK signaling, elevates angiogenic cytokines, and promotes cancer invasion and metastasis. Restoration of wild-type MIPOL1 enhances RhoB activity and suppresses tumor progression both in vitro and in xenograft mouse models. (PMID: 31609475)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MIPOL1 antibody 14158-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
