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TOM20 Polyclonal antibody

TOM20 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (7)

Applications

FC, IF, IHC, IP, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 11802-1-AP

Print datasheet

Synonyms

Cell and organelle markers, KIAA0016, MAS20, Mitochondria Marker, Mitochondrial Marker, Mitochondrion Marker, MOM19, tom 20, TOM20, TOMM20



经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, SH-SY5Y cells, mouse kidney tissue, rat kidney tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inmouse liver tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHUVEC cells, HepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11802-1-AP targets TOM20 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, IF, IHC, IP, WB, ELISA
Cited Applications CoIP, IF, IHC, IP, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, chicken, cattle, monkey, rabbit, zebrafish, hamster, pig
Immunogen TOM20 fusion protein Ag2378 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name translocase of outer mitochondrial membrane 20 homolog (yeast)
Synonyms Cell and organelle markers, KIAA0016, MAS20, Mitochondria Marker, Mitochondrial Marker, Mitochondrion Marker, MOM19, tom 20, TOM20, TOMM20
Calculated Molecular Weight 145 aa, 16 kDa
Observed Molecular Weight 16 kDa
GenBank Accession NumberBC000882
Gene Symbol TOMM20
Gene ID (NCBI) 9804
RRIDAB_2207530
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15388
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Background

 TOM20 (KIAA0016) belongs to the Tom family. It is a subunit of the mitochondrial import receptor (PMID: 7584026), whose main role is to translocate cytosolically synthesized mitochondrial proteins through the outer mitochondrial membrane and subsequently facilitate the movement of proteins into the TOM40 translocation pore complex (PMID: 21173275).

 What is the molecular weight of TOM20? 

 It is a short 16.3 kDa protein containing several highly conserved regions, including the transmembrane segment, the ligand-binding domain, and flexible segments at the N terminus and the C terminus of the protein crucial for its function (PMID: 15733919).

 What is the subcellular localization of TOM20?

 It specifically localizes in the mitochondrial outer membrane. In malignant cells, strong granular staining in the cytoplasm has been observed.

 What is the tissue specificity of TOM20?

 It is ubiquitously expressed in various tissues, with a particularly high expression in the brain, thyroid, and pancreas.

 What is the function of TOM20 in the mitochondrial membrane?

 Mitochondrial preproteins are generally synthesized in the cytoplasm with signal or targeting sequences, which are recognized by specific receptors on the outer mitochondrial membrane. TOM20, as one of the components of the TOM40 complex, specifically recognizes pre-sequences on target proteins or their unfolded forms. In addition to its translocase activity, TOM20 may act as a chaperone preventing these proteins from aggregation at the surface of mitochondria (PMID: 14699115).

 What is TOM20's involvement in disease?

 Diseases linked to the misregulation of TOM20 include Perry Syndrome and neurodegeneration with brain iron accumulation 2A. Numerous studies also associate deregulation of TOM20 with an array of mitochondrial dysfunctions and mitophagy defects (PMIDs: 30254015, 30160596). 


实验方案

Product Specific Protocols
WB protocol for TOM20 antibody 11802-1-APDownload protocol
IHC protocol for TOM20 antibody 11802-1-APDownload protocol
IF protocol for TOM20 antibody 11802-1-APDownload protocol
IP protocol for TOM20 antibody 11802-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Nature

CLSTN3β enforces adipocyte multilocularity to facilitate lipid utilization

Authors - Kevin Qian
humanWB

Cell Res

Mitochondria-localized cGAS suppresses ferroptosis to promote cancer progression

Authors - Shiqiao Qiu
mouseWB

Nature

BCAA catabolism in brown fat controls energy homeostasis through SLC25A44.

Authors - Takeshi Yoneshiro
mouseIF

Nature

Low-dose metformin targets the lysosomal AMPK pathway through PEN2.

Authors - Teng Ma
humanWB

Science

Comparative host-coronavirus protein interaction networks reveal pan-viral disease mechanisms.

Authors - David E Gordon
humanWB,IF

Cell

An in vitro system to silence mitochondrial gene expression.

Authors - Luis Daniel Cruz-Zaragoza