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验证数据展示

  • WB analysis using 11802-1-AP (same clone as 11802-1-PBS)

    WB analysis using 11802-1-AP (same clone as 11802-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11802-1-AP (TOM20 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11802-1-PBS in a different storage buffer formulation.

  • IF Staining of HUVEC using 11802-1-AP (same clone as 11802-1-PBS)

    IF Staining of HUVEC using 11802-1-AP (same clone as 11802-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using TOM20 antibody (11802-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 11802-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 11802-1-AP (same clone as 11802-1-PBS)

    WB analysis of HEK-293 using 11802-1-AP (same clone as 11802-1-PBS)

    WB result of TOM20 antibody (11802-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TOM20 transfected HEK-293 cells. This data was developed using the same antibody clone with 11802-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 11802-1-AP (same clone as 11802-1-PBS)

    IHC staining of mouse liver using 11802-1-AP (same clone as 11802-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11802-1-AP (TOM20 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11802-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TOMM20 antibody included
  • IHC staining of mouse liver using 11802-1-AP (same clone as 11802-1-PBS)

    IHC staining of mouse liver using 11802-1-AP (same clone as 11802-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11802-1-AP (TOM20 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11802-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TOMM20 antibody included
  • IP experiment of HeLa using 11802-1-AP (same clone as 11802-1-PBS)

    IP experiment of HeLa using 11802-1-AP (same clone as 11802-1-PBS)

    IP result of anti-TOM20 (IP:11802-1-AP, 4ug; Detection:11802-1-AP 1:6000) with HeLa cells lysate 1085 ug. This data was developed using the same antibody clone with 11802-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 11802-1-AP (same clone as 11802-1-PBS)

    IF Staining of HepG2 using 11802-1-AP (same clone as 11802-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TOM20 antibody (11802-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 11802-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 11802-1-AP (same clone as 11802-1-PBS)

    FC experiment of HeLa using 11802-1-AP (same clone as 11802-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug TOM20 Polyclonal antibody (11802-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019). This data was developed using the same antibody clone with 11802-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

TOM20 Polyclonal antibody, PBS Only

TOM20 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Cat No. 11802-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, chicken

应用

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

别名

TOMM20, KIAA0016, MAS20, MOM19, tom 20

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy TOMM20 Ready-To-Use IHC Kit. TOMM20 primary antibody included.

产品信息

11802-1-PBS targets TOM20 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat, chicken samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
经测试反应性 human, mouse, rat, chicken
免疫原

CatNo: Ag2378

Product name: Recombinant human TOM20 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-145 aa of BC000882

Sequence: MVGRNSAIAAGVCGALFIGYCIYFDRKRRSDPNFKNRLRERRKKQKLAKERAGLSKLPDLKDAEAVQKFFLEEIQLGEELLAQGEYEKGVDHLTNAIAVCGQPQQLLQVLQQTLPPPVFQMLLTKLPTISQRIVSAQSLAEDDVE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 translocase of outer mitochondrial membrane 20 homolog (yeast)
别名 TOMM20, KIAA0016, MAS20, MOM19, tom 20
计算分子量 145 aa, 16 kDa
观测分子量 16 kDa
GenBank蛋白编号BC000882
基因名称 TOM20
Gene ID (NCBI) 9804
RRIDAB_2207530
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ15388
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Background

 TOM20 (KIAA0016) belongs to the Tom family. It is a subunit of the mitochondrial import receptor (PMID: 7584026), whose main role is to translocate cytosolically synthesized mitochondrial proteins through the outer mitochondrial membrane and subsequently facilitate the movement of proteins into the TOM40 translocation pore complex (PMID: 21173275).

 What is the molecular weight of TOM20? 

 It is a short 16.3 kDa protein containing several highly conserved regions, including the transmembrane segment, the ligand-binding domain, and flexible segments at the N terminus and the C terminus of the protein crucial for its function (PMID: 15733919).

 What is the subcellular localization of TOM20?

 It specifically localizes in the mitochondrial outer membrane. In malignant cells, strong granular staining in the cytoplasm has been observed.

 What is the tissue specificity of TOM20?

 It is ubiquitously expressed in various tissues, with a particularly high expression in the brain, thyroid, and pancreas.

 What is the function of TOM20 in the mitochondrial membrane?

 Mitochondrial preproteins are generally synthesized in the cytoplasm with signal or targeting sequences, which are recognized by specific receptors on the outer mitochondrial membrane. TOM20, as one of the components of the TOM40 complex, specifically recognizes pre-sequences on target proteins or their unfolded forms. In addition to its translocase activity, TOM20 may act as a chaperone preventing these proteins from aggregation at the surface of mitochondria (PMID: 14699115).

 What is TOM20's involvement in disease?

 Diseases linked to the misregulation of TOM20 include Perry Syndrome and neurodegeneration with brain iron accumulation 2A. Numerous studies also associate deregulation of TOM20 with an array of mitochondrial dysfunctions and mitophagy defects (PMIDs: 30254015, 30160596). 


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