Speedy™ Mouse CTLA-4 One-Step ELISA Kit
Sensitivity
3.6 pg/mL
Range
46.88-3000 pg/mL
Reactivity
Mouse
Validation Data Gallery
Product Information
| Product name | Speedy™ Mouse CTLA-4 One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Tissue lysate 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 3.6 pg/mL |
| Range | 46.88-3000 pg/mL |
| Reactivity | Mouse |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 12477 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Mouse serum | 110% | 91%-123% |
| Tissue lysate | 91% | 84%-98% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,887.9 | 43.0 | 2.3 |
| 2 | 8 | 410.6 | 4.0 | 1.0 |
| 3 | 8 | 210.9 | 3.0 | 1.4 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 4.8 | 0.2 | 3.3 |
| 2 | 16 | 1.1 | 0.0 | 2.7 |
| 3 | 16 | 0.6 | 0.0 | 1.8 |
Background Information
Cytotoxic T-lymphocyte-associated antigen 4 (CTLA-4) is a co-inhibitory receptor that plays a crucial role in regulating T-cell activation and maintaining immune tolerance and delivers inhibitory signals that suppress T-cell responses, thereby preventing autoimmune diseases. It is expressed on activated T cells and regulatory T cells (Tregs), which mediates its inhibitory function by competing with CD28 for binding to CD80/CD86, depleting these ligands from antigen-presenting cells (APCs) and dendritic cells (DCs), and delivering inhibitory signals via its intracellular tail. CTLA-4 exists in two forms: membrane-bound CTLA-4 (mCTLA-4) and soluble CTLA-4 (sCTLA-4). mCTLA-4 is a transmembrane protein localized on the surface of T cells and Tregs, where it interacts with CD80 and CD86 on APCs. sCTLA-4 lacks the transmembrane domain and is released into the extracellular environment, where it can modulate immune responses.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Ctla4, Cd152, Ctla 4, Ly 56 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
2、我没有恒温振荡器，这会对实验时间和结果产生影响吗？
如果您没有恒温振荡器，完成这个实验大约需要 2.5 小时。在加入抗体混合物后，我们建议您将板条37℃下静置孵育2小时。在加入 TMB 后，您只需让板条37℃下静置孵育15分钟即可。我们的研发人员通过对比实验已经证明，尽管信号会略有降低，但您仍然可以获得有效的实验数据。
