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验证数据展示

  • FC experiment of Transfected HEK-293T using 98255-1-RR (same clone as 98255-1-PBS)

    FC experiment of Transfected HEK-293T using 98255-1-RR (same clone as 98255-1-PBS)

    1x10^6 HEK-293T cells or IL-4 transfected HEK-293T cells were intracellularly stained with 0.25 ug Anti-Rat IL-4 Rabbit Recombinant Antibody (98255-1-RR, Clone:241249H7) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 98255-1-PBS in a different storage buffer formulation.

  • FC experiment of Transfected HEK-293T using 98255-1-RR (same clone as 98255-1-PBS)

    FC experiment of Transfected HEK-293T using 98255-1-RR (same clone as 98255-1-PBS)

    1x10^6 HEK-293T cells (left) or IL-4 transfected HEK-293T cells (right) were intracellularly stained with 0.25 ug Anti-Rat IL-4 Rabbit Recombinant Antibody (98255-1-RR, Clone:241249H7) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (red), or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 98255-1-PBS in a different storage buffer formulation.

Anti-Rat IL-4 Rabbit Recombinant Antibody, PBS Only

IL-4 Uni-rAb® Recombinant Antibody for FC (Intra)
Cat No. 98255-1-PBS

产品说明书

CloneNo. 241249H7

宿主/亚型

Rabbit / IgG

种属反应性

rat

应用

FC (Intra)

别名

241249H7, B-cell growth factor 1, B-cell IGG differentiation factor, B-cell stimulatory factor 1, BSF-1

缓冲液配方:  PBS Only
PBS, Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

98255-1-PBS targets IL-4 in FC (Intra) applications and shows reactivity with rat samples.

经测试应用 FC (Intra) Application Description
经测试反应性 rat
免疫原

CatNo: Eg1113

Product name: Recombinant Rat IL-4 protein (His Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-6*HIS

Domain: 25-147 aa of NM_201270.1

Sequence: CNDSPLREIINTLNQVTEKGTPCTEMFVPDVLTATRNTTENELICRASRVLRKFYFPRDVPPCLKNKSGVLGELRKLCRGVSGLNSLRSCTVNESTLTTLKDFLESLKSILRGKYLQSCTSMS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 interleukin 4
别名 241249H7, B-cell growth factor 1, B-cell IGG differentiation factor, B-cell stimulatory factor 1, BSF-1
计算分子量16kDa
GenBank蛋白编号NM_201270.1
基因名称 IL-4
Gene ID (NCBI) 287287
RRIDAB_3745922
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP20096
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

IL-4 is a cytokine produced by CD4+ T cells in response to helminthes and other extracellular parasites. It promotes the proliferation and differentiation of antigen presenting cells. IL-4 also plays a pivotal role in antibody isotype switching and stimulates the production of IgE. This cytokine has been applied in the treatment of autoimmune disorder like multiple myeloma, cancer, psoriasis, and arthritis. IL-4 has also been extensively applied to inhibit detrimental effect of Th1. It may promote the growth of epithelial tumors by mediating increased proliferation and survival.

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