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验证数据展示

  • FC experiment of Transfected HEK-293T using 98567-3-RR (same clone as 98567-3-PBS)

    FC experiment of Transfected HEK-293T using 98567-3-RR (same clone as 98567-3-PBS)

    1x10^6 HEK-293T cells (left) or IL-28B transfected HEK-293T cells (right) were stained with 0.25 ug Anti-Mouse IL-28B Rabbit RecAb (98567-3-RR, Clone: 250563C9) and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 98567-3-PBS in a different storage buffer formulation.

  • FC experiment of Transfected HEK-293T using 98567-3-RR (same clone as 98567-3-PBS)

    FC experiment of Transfected HEK-293T using 98567-3-RR (same clone as 98567-3-PBS)

    1x10^6 HEK-293T cells (left) or IL-28B transfected HEK-293T cells (right) were stained with 0.25 ug Anti-Mouse IL-28B Rabbit RecAb (98567-3-RR, Clone: 250563C9) (red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9)(blue), and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 98567-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 98567-3-RR

    Affinity and Kinetic Characterization of 98567-3-RR

    Biolayer interferometry (BLl) kinetic assays of 98567-3-RR against Mouse IL-28B were performed. The affinity constant is below 1 pM.

Anti-Mouse IL-28B Rabbit Recombinant Antibody, PBS Only

IL-28B Uni-rAb® Recombinant Antibody for FC (Intra)
Cat No. 98567-3-PBS

产品说明书

CloneNo. 250563C9

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

FC (Intra)

别名

Ifnl3, IFN-lambda-3, Il28, Il28b, Interferon lambda-3

缓冲液配方:  PBS Only
PBS, Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

98567-3-PBS targets IL-28B in FC (Intra) applications and shows reactivity with mouse samples.

经测试应用 FC (Intra) Application Description
经测试反应性 mouse
免疫原

CatNo: Eg3146

Product name: Recombinant Mouse IL-28B/ IFNL3 protein (rFc Tag)

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 20-193 aa of NM_177396.1

Sequence: DPVPRATRLPVEAKDCHIAQFKSLSPKELQAFKKAKGAIEKRLLEKDMRCSSHLISRAWDLKQLQVQERPKALQAEVALTLKVWENINDSALTTILGQPLHTLSHIHSQLQTCTQLQATAEPKPPSRRLSRWLHRLQEAQSKETPGCLEDSVTSNLFQLLLRDLKCVASGDQCV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 interleukin 28B
别名 Ifnl3, IFN-lambda-3, Il28, Il28b, Interferon lambda-3
计算分子量22 kDa
GenBank蛋白编号NM_177396.1
基因名称 Il28b
Gene ID (NCBI) 338374
RRIDAB_3746184
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ8CGK6
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

IL-28B is a type III IFN, which shares many of the biological effects of type I IFNs but may have fewer side effects due to a more selective receptor distribution. It interacts with a heterodimeric class II cytokine receptor that consists of interleukin 10 receptor, beta (IL10RB) and interleukin 28 receptor, alpha (IL28RA) (PMID: 20712453;12469119;16539846).

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