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验证数据展示

  • FC experiment of mouse bone marrow cells using 98532-2-RR

    FC experiment of mouse bone marrow cells using 98532-2-RR

    1x10^6 mouse bone marrow cells were surface stained with 0.25 ug Anti-Mouse IL-17RA Rabbit RecAb (98532-2-RR, Clone: 250349C6) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Mouse Ly-6G/Ly-6C (Gr-1). Cells were not fixed.

  • FC experiment of mouse bone marrow cells using 98532-2-RR

    FC experiment of mouse bone marrow cells using 98532-2-RR

    1x10^6 mouse bone marrow cells were surface stained with 0.25 ug Anti-Mouse IL-17RA Rabbit RecAb (98532-2-RR, Clone: 250349C6) (red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

Anti-Mouse IL-17RA Rabbit Recombinant Antibody

IL-17RA Uni-rAb® Recombinant Antibody for FC
Cat No. 98532-2-RR

产品说明书

CloneNo. 250349C6

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

FC

别名

CD217, IL-17 receptor A, Il17r, Il17ra, Interleukin-17 receptor A

缓冲液配方:  PBS, Azide
PBS, Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive FC detected inmouse bone marrow cells

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98532-2-RR targets IL-17RA in FC applications and shows reactivity with mouse samples.

经测试应用 FC Application Description
经测试反应性 mouse
免疫原

CatNo: Eg2584

Product name: Recombinant Mouse IL-17RA protein (rFc Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 32-322 aa of NM_008359.2

Sequence: SPRLLDFPAPVCAQEGLSCRVKNSTCLDDSWIHPKNLTPSSPKNIYINLSVSSTQHGELVPVLHVEWTLQTDASILYLEGAELSVLQLNTNERLCVKFQFLSMLQHHRKRWRFSFSHFVVDPGQEYEVTVHHLPKPIPDGDPNHKSKIIFVPDCEDSKMKMTTSCVSSGSLWDPNITVETLDTQHLRVDFTLWNESTPYQVLLESFSDSENHSCFDVVKQIFAPRQEEFHQRANVTFTLSKFHWCCHHHVQVQPFFSSCLNDCLRHAVTVPCPVISNTTVPKPVADYIPLW

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 interleukin 17 receptor A
别名 CD217, IL-17 receptor A, Il17r, Il17ra, Interleukin-17 receptor A
计算分子量98kDa
GenBank蛋白编号NM_008359.2
基因名称 Il17ra
Gene ID (NCBI) 16172
RRIDAB_3746159
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ60943
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

IL-17 is a proinflammatory cytokine. IL-17 can signal as homodimers or heterodimers through a heterodimeric receptor formed by IL-17 receptor A (IL-17RA) and IL-17 receptor C (IL-17RC) subunits. IL-17RA is ubiquitously expressed in both the epithelial and hematopoietic cell compartments. IL-17/IL-17RA signaling plays an important physiological role in regulating the mucosal host defense against invading microorganisms, but it can also play pathogenic roles in several inflammatory or autoimmune conditions; furthermore, it has been described as pro-tumorigenic in several cancer types.

实验方案

Product Specific Protocols
FC protocol for IL-17RA antibody 98532-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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