Human/Mouse/Rat CKB ELISA Kit
Sensitivity
0.18 ng/mL
Range
1.56-100 ng/mL
Reactivity
Human/Mouse/Rat
Validation Data Gallery
Product Information
KE00489 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human/mouse/rat CKB ELISA kit is to be used to detect and quantify protein levels of endogenous human/mouse/rat CKB. The assay recognizes human/mouse/rat CKB. An antibody specific for human/mouse/rat CKB has been pre-coated onto the microwells. The human/mouse/rat CKB protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human/mouse/rat CKB is added to detect the captured human/mouse/rat CKB protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human/Mouse/Rat CKB ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate, Tissue lysate 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.18 ng/mL |
| Range | 1.56-100 ng/mL |
| Reactivity | Human/Mouse/Rat |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 1152 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Tissue lysate | 77% | 72%-87% |
| Cell lysate | 112% | 87%-125% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 43.14 | 1.89 | 4.38 |
| 2 | 8 | 10.78 | 1.00 | 9.29 |
| 3 | 8 | 3.29 | 0.18 | 5.39 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 45.41 | 2.24 | 4.94 |
| 2 | 16 | 10.89 | 0.98 | 9.02 |
| 3 | 16 | 3.25 | 0.25 | 7.81 |
Background Information
CKB (Creatine kinase B-type), also named as B-CK (Brain creatine kinase) and CKBB, is a member of the ATP:guanido phosphotransferase protein family. It is a cytoplasmic enzyme involved in energy homeostasis. CKBB reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. CK isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. CK MB consists of a dimer of nonidentical chains. MM being the major form in skeletal muscle and myocardium, MB existing in myocardium, and BB exists in many tissues, especially brain.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CKB,KCRB,B CK,B-CK,CKBB |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
