Human CD71 ELISA Kit
Sensitivity
0.2 ng/mL
Range
1.56-100 ng/mL
Reactivity
Human
Cat no : KE00081
Synonyms
CD71, p90, sTfR, T9, TFR, TFR1, TFRC, TR, Transferrin receptor protein 1, TRFR
Validation Data Gallery
Product Information
KE00081 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The CD71 ELISA kit is to be used to detect and quantify protein levels of endogenous CD71. The assay recognizes human CD71. A polyclonal antibody specific for CD71 has been pre-coated onto the microwells. The CD71 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody specific for CD71 is added to detect the captured CD71 protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.
| Product name | Human CD71 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.2 ng/mL |
| Range | 1.56-100 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 7037 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Plasma | 92% | 83%-101% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | ? ug/ml | ? | ?% |
| 2 | 20 | ? ug/ml | ? | ?% |
| 3 | 20 | ? ug/ml | ? | ?% |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | ? ug/ml | ? | ?% |
| 2 | 20 | ? ug/ml | ? | ?% |
| 3 | 20 | ? ug/ml | ? | ?% |
Background Information
CD71, also known as transferrin receptor protein 1 (TfR1), is a transmembrane glycoprotein which mediates cellular uptake of iron from transferrin, a plasma protein which transports iron in the circulation. CD71 is a homodimer of two identical transmembrane subunits linked by two disulfide bonds. CD71 is almost ubiquitously expressed, with highest expression levels on some cells and tissues, including immature erythroid cells, placental tissue, and rapidly dividing cells. Soluble CD71 present in the circulation is the truncated form of the cell surface receptor. The concentration of serum CD71 has been suggested to provide a sensitive measure of iron depletion.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD71, p90, sTfR, T9, TFR, TFR1, TFRC, TR, Transferrin receptor protein 1, TRFR |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
