验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HepG2 cells, U-937 cells, Raji cells, human white blood, NCCIT cells, pig spleen tissue, Calu-1 cells, HEK-293 cells, Jurkat cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human tonsillitis tissue, human liver cancer tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human tonsillitis tissue |
| Positive IF/ICC detected in | Raji cells |
| Positive FC detected in | Raji cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:10000 |
| Immunofluorescence (IF)-P | IF-P : 1:400-1:1600 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66013-1-Ig targets HLA class I ABC in WB, IHC, IF/ICC, IF-P, FC, IP, ELISA applications and shows reactivity with human, pig samples.
| 经测试应用 | WB, IHC, IF/ICC, IF-P, FC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, FC, IP |
| 经测试反应性 | human, pig |
| 文献引用反应性 | human, pig |
| 免疫原 |
CatNo: Ag7488 Product name: Recombinant human HLA class I (HLA-A) protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 29-302 aa of BC003069 Sequence: MRYFFTSVSRPGRGEPRFIAVGYVDDTQFVRFDSDAASQKMEPRAPWIEQEGPEYWDQETRNMKAHSQTDRANLGTLRGYYNQSEDGSHTIQIMYGCDVGPDGRFLRGYRQDAYDGKDYIALNEDLRSWTAADMAAQITKRKWEAVHAAEQRRVYLEGRCVDGLRRYLENGKETLQRTDPPKTHMTHHPISDHEATLRCWALGFYPAEITLTWQRDGEDQTQDTELVETRPAGDGTFQKWAAVVVPSGEEQRYTCHVQHEGLPKPLTLRWELSS 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | major histocompatibility complex, class I, A |
| 别名 | HLA-A, 5C5B7, HLA A, HLA class I, HLA class I (HLA-A) |
| 计算分子量 | 41 kDa |
| 观测分子量 | 40-44 kDa |
| GenBank蛋白编号 | BC003069 |
| 基因名称 | HLA-A |
| Gene ID (NCBI) | 3105 |
| RRID | AB_11042593 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P04439 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I (HLA-A, B and C) and class II (HLA-D). This class I molecules are polymorphic membrane glycoproteins composed of a heavy (alpha) chain (44 kDa) which is encoded by a HLA class I gene (HLA-A, B or C), and β2-microglobulin light (beta) chain (12 kDa). They are involved in the presentation of foreign antigens to the immune system. (PMID: 667938; 3375250)
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for HLA class I ABC antibody 66013-1-Ig | Download protocol |
| IF protocol for HLA class I ABC antibody 66013-1-Ig | Download protocol |
| IHC protocol for HLA class I ABC antibody 66013-1-Ig | Download protocol |
| IP protocol for HLA class I ABC antibody 66013-1-Ig | Download protocol |
| WB protocol for HLA class I ABC antibody 66013-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cancer IFITM3 enhances immunosensitivity via MHC-I regulation and is associated with the efficacy of anti-PD-1/-L1 therapy in SCLC. | ||
Adv Sci (Weinh) Engineered Xenogeneic Bone Scaffold with IL-10 Nanodelivery System: Immunomodulation and BMSC Fate Programming for Skull Defect Repair. | ||
Adv Sci (Weinh) Genetic Ablation and Multi-Omics Profiling Reveal CEP55 as a Key Driver of Tumorigenesis in Diverse Cancer Models. | ||
Cell Death Dis AZIN1-dependent polyamine synthesis accelerates tumor cell cycle progression and impairs effector T-cell function in osteosarcoma | ||
Int J Biol Sci Prolonged DEHP exposure enhances the stemness and metastatic potential of TNBC cells in an MSI2-dependent manner | ||
Proc Natl Acad Sci U S A Inhibition of major histocompatibility complex-I antigen presentation by sarbecovirus ORF7a proteins |
