HRP-conjugated GFP tag Monoclonal antibody

GFP tag Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG2a

Reactivity

recombinant protein, aequorea victoria

Applications

WB and More (2)

Conjugate

HRP Fluorescent Dye

CloneNo.

1E10H7

Cat No : HRP-66002

Print datasheet

Synonyms

CFP, eGFP, eYFP, GFP, GFP tag, HRP CFP, HRP eGFP, HRP GFP, HRP YFP, YFP



经过测试的应用

Positive WB detected inRecombinant protein

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Sample-dependent, check data in validation data gallery

产品信息

HRP-66002 targets GFP tag in WB, IF, CoIP applications and shows reactivity with recombinant protein, aequorea victoria samples.

Tested Applications WB
Cited Applications CoIP, IF, WB
Tested Reactivity recombinant protein, aequorea victoria
Immunogen Recombinant Protein 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name GFP tag
Synonyms CFP, eGFP, eYFP, GFP, GFP tag, HRP CFP, HRP eGFP, HRP GFP, HRP YFP, YFP
Calculated molecular weight 26 kDa
Observed molecular weight-
GenBank accession numberU73901
Gene symbol
Gene ID (NCBI)
RRIDAB_2883834
Conjugate HRP Fluorescent Dye
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Green fluorescence protein(GFP) is a protein composed of 238 amino acid residues(26.9kDa) derived from the Jellyfish Aequorea victoria, which emits green light(emission peak at 509nm) when excited by blue light(excitation peak at 395nm). GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. EGFP contains the double-amino-acid substitutions Phe-64 to Leu and Ser-65 to Thr(previously published as GFPmut1; PMID: 8707053). In contrast to wtGFP, EGFP has a single, strong, red-shifted excitation peak at 488nm. GFPmut1 fluoresces 35-fold more intensely than wtGFP when excited at 488nm, due to an increase in its extinction coefficient(Em). The antibody recognizes the GFP-tag, eGFP tag, eYFP tag, CFP tag or YFP tag fused to either the amino- or carboxy-terminus of targeted proteins in transfected mammalian cells. This antibody is conjugated with HRP.


Note: Do not add Azium (Sodium Azide or Smite) into the dilution buffer. Azium is the HRP inhibitor which decreases the enzymatic activity of HRP.


发表文章

SpeciesApplicationTitle
WB

Cell

Human T-bet Governs Innate and Innate-like Adaptive IFN-γ Immunity against Mycobacteria.

Authors - Rui Yang
WB

Mol Neurodegener

Islet amyloid polypeptide cross-seeds tau and drives the neurofibrillary pathology in Alzheimer's disease.

Authors - Guoxin Zhang

Elife

A picorna-like virus suppresses the N-end rule pathway to inhibit apoptosis.

Authors - Zhaowei Wang
WB

Elife

Amphiphysin I cleavage by asparagine endopeptidase leads to tau hyperphosphorylation and synaptic dysfunction.

Authors - Xingyu Zhang

Plant Physiol

Upstream Open Reading Frame and Phosphate-Regulated Expression of Rice OsNLA1 Controls Phosphate Transport and Reproduction.

Authors - Shu-Yi Yang
WB,IF

Front Microbiol

The MERS-CoV N Protein Regulates Host Cytokinesis and Protein Translation via Interaction With EF1A.

Authors - Lin Zhu