GFP tag Monoclonal antibody

GFP tag Monoclonal Antibody for WB, IP, IF/ICC, FC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

recombinant protein and More (7)

Applications

WB, IP, IF/ICC, FC, ELISA and More (4)

Conjugate

Unconjugated

CloneNo.

1E10H7

Cat No : 66002-1-Ig

Print datasheet

Synonyms

CFP, eGFP, eYFP, GFP, GFP tag, GFP-tag, YFP



经过测试的应用

Positive WB detected inGFP transgenic mouse brain tissue, Recombinant protein
Positive IP detected inTransfected HEK-293 cells
Positive IF detected inTransfected HEK-293 cells
Positive FC detected inTransfected HEK-293 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®594 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:400-1:1600
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66002-1-Ig targets GFP tag in WB, IP, IF, FC, RIP, IHC, CoIP, ChIP, ELISA applications and shows reactivity with recombinant protein samples.

Tested Applications WB, IP, IF/ICC, FC, ELISA
Cited ApplicationsWB, IP, IF, FC, RIP, IHC, CoIP, ChIP, ELISA
Tested Reactivity recombinant protein
Cited Reactivityhuman, mouse, rat, zebrafish, hamster, pig, duck
Immunogen GFP tag fusion protein Ag2128 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name GFP tag
Synonyms CFP, eGFP, eYFP, GFP, GFP tag, GFP-tag, YFP
Calculated Molecular Weight 26 kDa
GenBank Accession NumberU73901
Gene Symbol
Gene ID (NCBI)
RRIDAB_11182611
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Green Fluorescent Proteins (GFPs) encompass a diverse range of proteins carrying a green chromophore, originating from various species and forming different protein lineages. 

Wildtype GFP consists of 238 amino acid residues (26.9 kDa). GFP was first identified in the jellyfish Aequorea victoria. It emits green light with a peak wavelength of 509 nm upon excitation by blue light at 395 nm.

When fused with other proteins, GFP serves as a versatile reporter protein e.g. for quantifying expression levels or facilitates visualization of subcellular localization through fluorescence microscopy.

This antibody is a mouse (IgG2a) monoclonal antibody against GFP reactive to GFP, eGFP, eYFP, YFP and CFP.


实验方案

Product Specific Protocols
WB protocol for GFP tag antibody 66002-1-IgDownload protocol
IF protocol for GFP tag antibody 66002-1-IgDownload protocol
IP protocol for GFP tag antibody 66002-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

Nature

The CoQ oxidoreductase FSP1 acts parallel to GPX4 to inhibit ferroptosis.

Authors - Kirill Bersuker
WB,IF

Cell Discov

Glc7/PP1 dephosphorylates histone H3T11 to regulate autophagy and telomere silencing in response to nutrient availability

Authors - Xinyu Zhang
WB

Nat Genet

Pathogenic SPTBN1 variants cause an autosomal dominant neurodevelopmental syndrome.

Authors - Margot A Cousin
IF

Cell Stem Cell

DUX-miR-344-ZMYM2-Mediated Activation of MERVL LTRs Induces a Totipotent 2C-like State.

Authors -

Nat Neurosci

Reversal of prolonged obesity-associated cerebrovascular dysfunction by inhibiting microglial Tak1.

Authors - Qing Shen
WB

Nat Biomed Eng

Restoration of dystrophin expression in mice by suppressing a nonsense mutation through the incorporation of unnatural amino acids.

Authors - Ningning Shi