验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, mouse brain tissue, SH-SY5Y cells, rat brain tissue |
| Positive IP detected in | SH-SY5Y cells |
| Positive IF-P detected in | mouse brain tissue |
| Positive IF-Fro detected in | mouse brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)-FRO | IF-FRO : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
55084-1-AP targets DRD2 in WB, IHC, IF-P, IF-Fro, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IF-P, IF-Fro, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | dopamine receptor D2 |
| 别名 | D(2) dopamine receptor, D2 dopamine receptor, D2DR, D2R, Dopamine D2 receptor |
| 计算分子量 | 51 kDa |
| 观测分子量 | 50-60 kDa |
| GenBank蛋白编号 | NM_016574 |
| 基因名称 | DRD2 |
| Gene ID (NCBI) | 1813 |
| RRID | AB_10859941 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P14416 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DRD2, also named D2DR and D2R, belongs to the G-protein coupled receptor 1 family. It is one of the five types (D1 to D5) of receptors for dopamine. The activity of DRD2 is mediated by G proteins which inhibit adenylyl cyclase. Defects in DRD2 are associated with dystonia type 11 (DYT11). The antibody is specific to DRD2.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for DRD2 antibody 55084-1-AP | Download protocol |
| IP protocol for DRD2 antibody 55084-1-AP | Download protocol |
| WB protocol for DRD2 antibody 55084-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Adv Mater Intranasal Delivery of Near-infrared and Magnetic Dual-response Nanospheres to Rapidly Produce Antidepressant-like and Cognitive Enhancement Effects | ||
Mol Neurodegener Pathological characteristics of axons and alterations of proteomic and lipidomic profiles in midbrain dopaminergic neurodegeneration induced by WDR45-deficiency | ||
Nat Commun Non-canonical interplay between glutamatergic NMDA and dopamine receptors shapes synaptogenesis | ||
Adv Sci (Weinh) Neuroligin 1 Regulates Autistic-Like Repetitive Behavior through Modulating the Activity of Striatal D2 Receptor-Expressing Medium Spiny Neurons | ||
J Immunother Cancer Pharmacologic targeting of the dopamine D2 receptor impacts the efficacy of immune checkpoint blockade in melanoma. |
