COL4A2-specific Polyclonal antibody
COL4A2-specific Polyclonal Antibody for IF-P, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
IF-P, IHC, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, mouse lung tissue, rat lung tissue |
| Positive IHC detected in | human kidney tissue, human breast hyperplasia tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | human kidney tissue |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IHC | See 21 publications below |
| IF | See 13 publications below |
产品信息
55131-1-AP targets COL4A2-specific in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IF-P, IHC, WB, ELISA |
| Cited Applications | IF, IHC |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, pig |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | collagen, type IV, alpha 2 |
| Synonyms | COL4A2, Collagen alpha 2(IV) chain, Collagen Type IV, collagen, type IV, alpha 2 |
| Calculated Molecular Weight | 168 kDa |
| Observed Molecular Weight | 200 kDa |
| GenBank Accession Number | NM_001846 |
| Gene Symbol | COL4A2 |
| Gene ID (NCBI) | 1284 |
| RRID | AB_2881273 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P08572 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for COL4A2-specific antibody 55131-1-AP | Download protocol |
| IHC protocol for COL4A2-specific antibody 55131-1-AP | Download protocol |
| IF protocol for COL4A2-specific antibody 55131-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
EBioMedicine Aspirin targets P4HA2 through inhibiting NF-κB and LMCD1-AS1/let-7g to inhibit tumour growth and collagen deposition in hepatocellular carcinoma. | ||
ACS Appl Mater Interfaces Novel Thermosensitive Hydrogel Promotes Spinal Cord Repair by Regulating Mitochondrial Function. | ||
Biomaterials Tumor-specific nitric oxide generator to amplify peroxynitrite based on highly penetrable nanoparticles for metastasis inhibition and enhanced cancer therapy. | ||
Biofabrication Engineering microvasculature by 3D bioprinting of prevascularized spheroids in photo-crosslinkable gelatin. | ||
Oxid Med Cell Longev Pretreatment with Roxadustat (FG-4592) Attenuates Folic Acid-Induced Kidney Injury through Antiferroptosis via Akt/GSK-3β/Nrf2 Pathway. |
