Enhancement of GFP signal with GFP-Booster Atto488: Comparison of signal intensity of a cell line stably expressing a nuclear GFP-fusion protein before and after GFP-Booster treatment.

Enhancement of GFP signal with GFP-Booster Atto488: Comparison of signal intensity of a cell line stably expressing a nuclear GFP-fusion protein before and after GFP-Booster treatment.

Confocal image of HeLa cells transiently transfected with Tom70-eGFP and immunostained with GFP-Booster Alexa Fluor 488 (green). Nuclei were stained with DAPI (blue). Scale bar, 10 μm. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Enhancement of RFP signal with RFP-Booster_Atto594. Comparison of signal intensity of a cell line stably expressing a nuclear RFP-fusion protein before and after RFP-Booster treatment. Microscope: InCell1000.

Immunostaining of Tubulin-GFP: HeLa cells low expressing Tubulin-GFP. Top: GFP signal of Tubulin-GFP (green) and DAPI stain (blue); Bottom: Tubulin-GFP detection by GFP-Booster coupled to Alexa Fluor 647.

Enhancement of GFP signal with GFP-Booster Atto488: Comparison of signal intensity of a cell line stably expressing a nuclear GFP-fusion protein before and after GFP-Booster treatment.

Enhancement of RFP signal with RFP-Booster_Atto594. Comparison of signal intensity of a cell line stably expressing a nuclear RFP-fusion protein before and after RFP-Booster treatment. Microscope: InCell1000.

HeLa cells transiently transfected with PCNA-mRFP and Tom70-eGFP were subjected to one-step immunostaining with RFP-Booster Alexa Fluor 568 (red) and GFP-Booster Alexa Fluor 488 (green). Scale bar, 10 μm. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.