IP sample detected with different secondary antibodies. Normal Rabbit IgG (30000-0-AP) as control.

IP sample detected with different secondary antibodies. Normal Rabbit IgG (30000-0-AP) as control.

1X10^6 Jurkat cells were intracellularly stained with 0.2 ug Anti-Rabbit IgG control (30000-0-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

1X10^6 K-562 cells were stained with 0.20ug Mouse IgG1 isotype control antibody (66360-1-Ig, red) and another control antibody (blue). Fixed with 90% MeOH.

Various lysates were subjected to SDS PAGE followed by western blot with 66360-1-Ig (Mouse IgG1 isotype control antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Various lysates were subjected to SDS PAGE followed by western blot with 66360-1-Ig (Mouse IgG1 isotype control antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Various lysates (50 ug/lane) were subjected to SDS PAGE followed by western blot with 66360-3-Ig (Mouse IgG2b isotype control antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

1X10^6 Jurkat cells were stained with 0.20ug Mouse IgG2b isotype control antibody (66360-3-Ig, red) or control antibody (blue). Fixed with 90% MeOH.

Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66360-3-Ig (Mouse IgG2b isotype control antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66360-3-Ig (Mouse IgG2b isotype control antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

1X10^6 BALB/c mouse splenocytes were surface stained with 0.25 ug APC Armenian Hamster IgG Isotype Control (APC-65210, Clone: PIP) (blue) or 0.25 ug APC Anti-Mouse CD3ε (APC-65060, Clone: 145-2C11) (red). Cells were not fixed.

1X10^6 human PBMCs were surface stained with 0.2 ug APC Anti-Mouse IgG1 Isotype Control (APC-65124, Clone:MOPC-21) (red), APC Anti-Human CD4 (green), or unstained (blue). Cells were not fixed. Lymphocytes were gated.

Various lysates (50 μg/lane) were subjected to SDS PAGE followed by western blot with 66360-2-Ig (Mouse IgG2a isotype control antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Mouse IgG2a isotype control (66360-2-Ig, Clone: 11A1B2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Cytochrome c Mouse McAb (66264-1-Ig, Clone: 2D8D11) (green) or unstained (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

1X10^6 human PBMCs were surface stained with 0.2 ug PE Anti-Mouse IgG2a Isotype Control (PE-65208, Clone:C1.18.4) (red), or PE Anti-Human CD3 (OKT3) (green), or unstained (blue). Cells were not fixed. Lymphocytes were gated.

1X10^6 human PBMCs were surface stained with 0.2 ug APC Anti-Mouse IgG2b Isotype Control (APC-65128, Clone:MPC-11) (red), APC Anti-Human CD20 (black), or unstained (blue). Cells were not fixed. Lymphocytes were gated.