Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PCNA antibody (10205-2-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL94-phalloidin (red), DAPI (Blue).

1X10^6 Jurkat cells were intracellularly stained with 0.2 ug Anti-Human PCNA (10205-2-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), and 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

WB result of PCNA antibody (10205-2-AP, 1:10000) with si-control and si-PCNA transfected HEK293 cells.

Various lysates were subjected to SDS PAGE followed by western blot with 10205-2-AP (PCNA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 10205-2-AP (PCNA antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

IP result of anti-PCNA (IP:10205-2-AP, 4ug; Detection:10205-2-AP 1:20000) with MCF-7 cells lysate 1880 ug.

Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10205-2-AP (PCNA antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Various lysates were subjected to SDS PAGE followed by western blot with 10205-2-AP (PCNA antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

Various lysates were subjected to SDS PAGE followed by western blot with 10205-2-AP (PCNA antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10205-2-AP (PCNA antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10205-2-AP (PCNA antibody) at dilution of 1:3000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PCNA antibody (10205-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using PCNA antibody (10205-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

E14.5 FFPE mouse heart stained for Desmin (green, Cat. No CL488-16520) and PCNA (red, Cat. No 60097-1-Ig). Desmin was conjugated to CoraLite-488 fluorescent dye and stains cardiomyocytes (heart muscle cells). PCNA stains proliferating cells, which are numerous in the developing heart. In this image, the four chambers of the heart can be easily visualized. Image credit: @Immunofluorescence on Instagram.

Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 60097-1-Ig (PCNA antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 60097-1-Ig (PCNA antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

HEK-293 cells were subjected to SDS PAGE followed by western blot with 60097-1-Ig (PCNA Antibody) at various dilution incubated at room temperature for 1.5 hours.

Western blot analysis of PCNA in various cell lines using Proteintech antibody 60097-1-Ig at a dilution of 1:10000 (exposed for 2 seconds) incubated at room temperature for 1.5 hours.

IP result of anti-PCNA (IP:60097-1-Ig, 4ug; Detection:60097-1-Ig 1:300) with HepG2 cells lysate 3000ug.

1X10^6 MCF-7 cells were stained with 0.2ug PCNA antibody (60097-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1500.

WB result of PCNA antibody (60097-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PCNA transfected HEK-293 cells.

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60097-1-Ig (PCNA antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 24036-1-AP (PCNA antibody) at dilution of 1:1600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

HEK-293 cells were subjected to SDS PAGE followed by western blot with 24036-1-AP (PCNA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human PCNA (24036-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 24036-1-AP (PCNA antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

IP result of anti-PCNA (IP:24036-1-AP, 4ug; Detection:24036-1-AP 1:1000) with HEK-293 cells lysate 2000ug.

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24036-1-AP (PCNA Antibody) at dilution of 1:400 (under 40x lens).

Various lysates were subjected to SDS PAGE followed by western blot with 24036-1-AP (PCNA antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

1X10^6 Jurkat cells were intracellularly stained with 0.2 ug Anti-Human PCNA (24036-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using Biotin-60097 (PCNA antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using Biotin-60097 (PCNA antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite® Plus 488 PCNA antibody (CL488-10205) at dilution of 1:200.

1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human PCNA (CL488-10205) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human PCNA (CL488-10205) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite®594 PCNA antibody (CL594-10205) at dilution of 1:200.

1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human PCNA (CL594-10205) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite®594 PCNA antibody (CL594-10205) at dilution of 1:200.

Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite®594 PCNA antibody (CL594-10205) at dilution of 1:200.

1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human PCNA (CL594-10205) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

HEK-293 cells were subjected to SDS PAGE followed by western blot with HRP-60097 (PCNA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Various lysates were subjected to SDS PAGE followed by western blot with CL750-60097 (PCNA antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.