Various lysates were subjected to SDS PAGE followed by western blot with 10197-1-AP (HDAC1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10197-1-AP (HDAC1 antibody) at dilution of 1:50 (under 40x lens).
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HDAC1 antibody (10197-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IP result of anti-HDAC1 (IP:10197-1-AP, 4ug; Detection:10197-1-AP 1:6000) with HeLa cells lysate 1600 ug.
1X10^6 HeLa cells were stained with 0.2ug HDAC1 antibody (10197-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
