Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16485-1-AP (CDC25C antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

1X10^6 K-562 cells were intracellularly stained with 0.2 ug Anti-Human CDC25C (16485-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH .

WB result of CDC25C antibody (16485-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CDC25C transfected HeLa cells.

Various lysates were subjected to SDS PAGE followed by western blot with 16485-1-AP (CDC25C antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

IP result of anti-CDC25C (IP:16485-1-AP, 4ug; Detection:16485-1-AP 1:1000) with K-562 cells lysate 3600ug.

Non-treated HeLa and nocodazole treated HeLa cells were subjected to SDS PAGE followed by western blot with 16485-1-AP (CDC25C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

Various lysates were subjected to SDS PAGE followed by western blot with 66912-1-Ig (CDC25C antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

1X10^6 K-562 cells were intracellularly stained with 0.2 ug Anti-Human CDC25C (66912-1-Ig, Clone:2B6B12) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH .

Various lysates were subjected to SDS PAGE followed by western blot with 66912-1-Ig (CDC25C antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

rat brain tissue were subjected to SDS PAGE followed by western blot with 12958-1-AP (RASGRF1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12958-1-AP (RASGRF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

IP result of anti-RASGRF1(IP:12958-1-AP, 4ug; Detection:12958-1-AP 1:600) with rat brain tissue lysate 1120 ug.

Various lysates were subjected to SDS PAGE followed by western blot with 66912-1-Ig (CDC25C antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66912-1-PBS in a different storage buffer formulation.

1X10^6 K-562 cells were intracellularly stained with 0.2 ug Anti-Human CDC25C (66912-1-Ig, Clone:2B6B12) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH . This data was developed using the same antibody clone with 66912-1-PBS in a different storage buffer formulation.

Various lysates were subjected to SDS PAGE followed by western blot with 66912-1-Ig (CDC25C antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66912-1-PBS in a different storage buffer formulation.

1X10^6 K-562 cells were intracellularly stained with 0.4 ug CoraLite®488 Anti-Human CDC25C (CL488-66912, Clone:2B6B12) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Non-treated and Forskolin treated HeLa cells were subjected to SDS PAGE followed by western blot with 82309-1-RR (Phospho-RASGRF1 (Ser927) antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with mTOR (66888-1-Ig) as loading control.

Non-treated and Forskolin treated HeLa cells were subjected to SDS PAGE followed by western blot with 82309-1-RR (Phospho-RASGRF1 (Ser927) antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with mTOR (66888-1-Ig) as loading control. This data was developed using the same antibody clone with 82309-1-PBS in a different storage buffer formulation.

pig cerebellum tissue were subjected to SDS PAGE followed by western blot with 67010-1-Ig (RASGRF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.