MCF-7 cells were subjected to SDS PAGE followed by western blot with 13762-1-AP (AMBRA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13762-1-AP (AMBRA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13762-1-AP (AMBRA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunofluorescent analysis of HepG2 cells using 13762-1-AP (AMBRA1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
