TUBB3-specific/TUJ1 Monoclonal antibody

TUBB3-specific/TUJ1 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, rabbit, chicken, pig

Applications

FC, IF, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1F8G10

Cat No : 66375-1-Ig

Print datasheet

Synonyms

beta 4, beta tubulin, MC1R, TUBB, TUBB3, TUBB3-specific, Tubulin beta 3 chain, Tubulin beta III, tubulin, beta 3, Tuj1, tuj1 antibody



经过测试的应用

Positive WB detected inSH-SY5Y cells, PC-12 cells, Neuro-2a cells, Pig brain, Rabbit brain, Rat brain Mouse brain, Chicken brain, human brain tissue
Positive IHC detected inhuman cerebellum tissue, mouse brain tissue, mouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inrat brain tissue, Retinal organoids, iPS cells, mouse brain tissue, human neuron
Positive FC detected inSH-SY5Y cells
Planning an IHC experiment? We recommend our IHCeasy TUBB3 Ready-To-Use IHC Kit. TUBB3 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:400-1:20000
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66375-1-Ig targets TUBB3-specific/TUJ1 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat, rabbit, chicken, pig samples.

Tested Applications FC, IF, IHC, WB, ELISA
Cited Applications FC, IF, IHC, WB
Tested Reactivity human, mouse, rat, rabbit, chicken, pig
Cited Reactivityhuman, mouse, rat, chicken
Immunogen Peptide 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name tubulin, beta 3
Synonyms beta 4, beta tubulin, MC1R, TUBB, TUBB3, TUBB3-specific, Tubulin beta 3 chain, Tubulin beta III, tubulin, beta 3, Tuj1, tuj1 antibody
Calculated Molecular Weight 55 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberNM_001197181
Gene Symbol TUBB3
Gene ID (NCBI) 10381
RRIDAB_2814998
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TUBB3, the class III β tubulin or Tuj1, is selectively expressed in testis and neurons of the central and peripheral nervous system. It has been widely used as a marker for neurons. Aberrant expression of TUBB3 has also been found in various tumors of non-neural origin and can be used as a biomarker for cancer aggressiveness and a marker for the tendency to respond poorly to chemotherapy. This antibody is specific to TUBB3 but not cross-react with other tubulin isoforms.

实验方案

Product Specific Protocols
WB protocol for TUBB3-specific/TUJ1 antibody 66375-1-IgDownload protocol
IHC protocol for TUBB3-specific/TUJ1 antibody 66375-1-IgDownload protocol
IF protocol for TUBB3-specific/TUJ1 antibody 66375-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Neuron

Posterior Neocortex-Specific Regulation of Neuronal Migration by CEP85L Identifies Maternal Centriole-Dependent Activation of CDK5.

Authors - Andrew Kodani
ratIF

Sci Total Environ

Ambient NO2 exposure induces migraine in rats: Evidence, mechanisms and interventions.

Authors - Shuzi Ye
ratIF

Curr Biol

Pyramidal neuron morphogenesis requires a septin network that stabilizes filopodia and suppresses lamellipodia during neurite initiation

Authors - Megan R Radler
mouseWB

Cell Rep

NLRP14 deficiency causes female infertility with oocyte maturation defects and early embryonic arrest by impairing cytoplasmic UHRF1 abundance

Authors - Wenzhe Zhang
humanIF

Theranostics

Chlorogenic acid effectively treats cancers through induction of cancer cell differentiation.

Authors - Shuai Huang
mouseWB

Carbohydr Polym

Silk fibroin nanofibrous scaffolds incorporated with microRNA-222 loaded chitosan nanoparticles for enhanced neuronal differentiation of neural stem cells.

Authors - Zehao Li