- Featured Product
- KD/KO Validated
Caspase 1/p20/p10 Polyclonal antibody
Caspase 1/p20/p10 Polyclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (4)
Applications
IF, IHC, WB, ELISA and More (1)
Conjugate
Unconjugated
660
验证数据展示
经过测试的应用
Positive WB detected in | Raji cells, HeLa cells, C6 cells, Jurkat cells, MCF-7 cells, NIH/3T3 cells, HepG2 cells, THP-1 cells, PC-12 cells, U-937 cells |
Positive IHC detected in | human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | C6 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:12000 |
Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
Immunofluorescence (IF) | IF : 1:10-1:100 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
22915-1-AP targets Caspase 1/p20/p10 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | IF, IHC, WB, ELISA |
Cited Applications | IF, IHC, IP, WB |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, monkey, pig, canine, bovine |
Immunogen | Caspase 1/p20/p10 fusion protein Ag11143 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta, convertase) |
Synonyms | CASP 1, CASP1, Caspase 1, Caspase 1/p20/p10, Caspase1, ICE, IL 1 beta converting enzyme, IL 1BC, IL1BC, IL1BCE, p10, p20, P45 |
Calculated Molecular Weight | 404 aa, 45 kDa |
Observed Molecular Weight | 45-47 kDa, 30 kDa, 35 kDa |
GenBank Accession Number | BC062327 |
Gene Symbol | CASP1 |
Gene ID (NCBI) | 834 |
RRID | AB_2876874 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P29466 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for Caspase 1/p20/p10 antibody 22915-1-AP | Download protocol |
IHC protocol for Caspase 1/p20/p10 antibody 22915-1-AP | Download protocol |
IF protocol for Caspase 1/p20/p10 antibody 22915-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Mol Cancer Metal-enriched HSP90 nanoinhibitor overcomes heat resistance in hyperthermic intraperitoneal chemotherapy used for peritoneal metastases | ||
ACS Nano Engineering Extracellular Vesicles Restore the Impaired Cellular Uptake and Attenuate Intervertebral Disc Degeneration. | ||
Mol Cell Serine synthesis sustains macrophage IL-1β production via NAD+-dependent protein acetylation | ||
Nat Commun REV-ERBα integrates colon clock with experimental colitis through regulation of NF-κB/NLRP3 axis. | ||
Theranostics Ablation of NLRP3 inflammasome attenuates muscle atrophy via inhibiting pyroptosis, proteolysis and apoptosis following denervation | ||
J Clin Invest Melatonin inhibits cytosolic mitochondrial DNA-induced neuroinflammatory signaling in accelerated aging and neurodegeneration. |