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Caspase 9/p35/p10 Polyclonal antibody
Caspase 9/p35/p10 Polyclonal Antibody for WB,IP,IF/ICC,FC,IHC,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (8)
Applications
WB,IP,IF/ICC,FC,IHC,ELISA and More (1)
Conjugate
Unconjugated
698
验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, HeLa cells, HL-60 cells, Staurosporine treated Jurkat cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human heart tissue, mouse lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
| Positive FC detected in | HepG2 cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®555 and CoraLite® Plus 647 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:300-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:200 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10380-1-AP targets Caspase 9/p35/p10 in WB, IP, IF, FC, RIP, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB,IP,IF/ICC,FC,IHC,ELISA |
| Cited Applications | WB, IF, RIP, IHC, ELISA |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat, goat, sheep, rabbit, zebrafish, hamster, pig, canine |
| Immunogen | Caspase 9/p35/p10 fusion protein Ag0404 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | caspase 9, apoptosis-related cysteine peptidase |
| Synonyms | APAF 3, APAF3, Apoptotic protease Mch 6, CASP 9, CASP9, Caspase 9, Caspase 9/p35/p10, CASPASE 9c, Caspase9, Caspase-9, ICE LAP6, ICE like apoptotic protease 6, MCH6 |
| Calculated Molecular Weight | 46 kDa |
| Observed Molecular Weight | 46 kDa, 30-39 kDa |
| GenBank Accession Number | BC002452 |
| Gene Symbol | CASP9 |
| Gene ID (NCBI) | 842 |
| RRID | AB_2068632 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P55211 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Caspase 9, apoptosis-related cysteine protease (CASP9, synonyms: MCH6, APAF3, APAF-3, ICE-LAP6, CASPASE-9c)is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. Caspase 9 is processed by APAF1; this step is thought to be one of the earliest in the caspase activation cascade. 10380-1-AP can recognize the pre- and cleaved- caspase 9. In recent years, the localization of caspase 9 was a focus of interest. Beside its cytoplasmic distribution, a very extensive localization study was done on rat brain tissue, where caspase 9 was found located predominantly in the nucleus and to a lesser extend in the cytoplasm [PMID: 15541731].
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Caspase 9/p35/p10 antibody 10380-1-AP | Download protocol |
| IHC protocol for Caspase 9/p35/p10 antibody 10380-1-AP | Download protocol |
| IF protocol for Caspase 9/p35/p10 antibody 10380-1-AP | Download protocol |
| IP protocol for Caspase 9/p35/p10 antibody 10380-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cancer hsa_circ_0007919 induces LIG1 transcription by binding to FOXA1/TET1 to enhance the DNA damage response and promote gemcitabine resistance in pancreatic ductal adenocarcinoma | ||
Nat Commun Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse model | ||
Acta Pharm Sin B Miltirone induces cell death in hepatocellular carcinoma cell through GSDME-dependent pyroptosis. | ||
Carbohydr Polym A multi-bioresponsive self-assembled nano drug delivery system based on hyaluronic acid and geraniol against liver cancer | ||
Adv Healthc Mater A ROS-Responsive Liposomal Composite Hydrogel Integrating Improved Mitochondrial Function and Pro-Angiogenesis for Efficient Treatment of Myocardial Infarction. | ||
Haematologica Therapeutic effects of the novel subtype-selective histone deacetylase (HDAC) inhibitor chidamide on myeloma-associated bone disease. |
