Various lysates were subjected to SDS PAGE followed by western blot with 10201-2-AP (ubiquitin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10201-2-AP (ubiquitin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ubiquitin antibody (10201-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ubiquitin (10201-2-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

ANTXR2 fusion protein Ag10201 SDS-PAGE

Immunofluorescent analysis of (4% PFA) fixed human pancreas cancer tissue using CoraLite® Plus 488 ubiquitin antibody (CL488-10201) at dilution of 1:200.

1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human ubiquitin (CL488-10201) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).