• Featured Product
  • KD/KO Validated

SLC7A11/xCT Polyclonal antibody

SLC7A11/xCT Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IP, IHC, ELISA and More (3)

Conjugate

Unconjugated

Cat No : 26864-1-AP

Print datasheet

Synonyms

Amino acid transport system xc, CCBR1, Cystine/glutamate transporter, SLC7A11, SLC7A11/xCT, xCT



经过测试的应用

Positive WB detected inA549 cells, U2OS cells, BxPC-3 cells, HepG2 cells
Positive IP detected inA549 cells, HepG2 cells
Positive IHC detected inhuman renal cell carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26864-1-AP targets SLC7A11/xCT in WB, IHC, IP, CoIP, RIP, ELISA, IF applications and shows reactivity with human samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsWB, IHC, IP, CoIP, RIP, IF
Tested Reactivity human
Cited Reactivityhuman, chicken, goat
Immunogen SLC7A11/xCT fusion protein Ag25431 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name solute carrier family 7, (cationic amino acid transporter, y+ system) member 11
Synonyms Amino acid transport system xc, CCBR1, Cystine/glutamate transporter, SLC7A11, SLC7A11/xCT, xCT
Calculated Molecular Weight 55 kDa
Observed Molecular Weight55 kDa, 35-40 kDa
GenBank Accession NumberBC012087
Gene Symbol SLC7A11
Gene ID (NCBI) 23657
RRIDAB_2880661
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UPY5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SLC7A11 (also known as xCT) is a membrane transporter involved in the uptake of cystine. It promotes glutathione synthesis through the uptake of cystine and the concomitant release of glutamate. This, in turn, protects cells from oxidative stress, maintains cellular redox balance, and prevents cell death induced by lipid peroxidation. SLC7A11 has been identified as a potential target for cancer therapeutics. It is overexpressed in multiple malignant tumors and is closely associated with the growth, prognosis, metastasis, and treatment response of various cancers including lung cancer. 26864-1-AP recognizes the 55 kDa and 35-40 kDa protein similar to the paper published (PMID: 32188872, 19156441).

实验方案

Product Specific Protocols
WB protocol for SLC7A11/xCT antibody 26864-1-APDownload protocol
IHC protocol for SLC7A11/xCT antibody 26864-1-APDownload protocol
IP protocol for SLC7A11/xCT antibody 26864-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Res

Mitochondria-localized cGAS suppresses ferroptosis to promote cancer progression

Authors - Shiqiao Qiu
humanWB

Drug Resist Updat

Upregulation of CoQ shifts ferroptosis dependence from GPX4 to FSP1 in acquired radioresistance

Authors - Xu Lin
humanWB

Nat Immunol

Glutathione peroxidase 4-regulated neutrophil ferroptosis induces systemic autoimmunity.

Authors - Pengchong Li
humanWB

ACS Nano

Engineering MMP-2 Activated Nanoparticles Carrying B7-H3 Bispecific Antibodies for Ferroptosis-Enhanced Glioblastoma Immunotherapy

Authors - Rangrang Fan
humanWB

Nat Commun

URI alleviates tyrosine kinase inhibitors-induced ferroptosis by reprogramming lipid metabolism in p53 wild-type liver cancers

Authors - Zhiwen Ding
humanWB,IHC,IF

Cell Rep Med

Disruption of MerTK increases the efficacy of checkpoint inhibitor by enhancing ferroptosis and immune response in hepatocellular carcinoma

Authors - Shun Wang
  • KD Validated