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验证数据展示

  • WB analysis using 60213-1-Ig (same clone as 60213-1-PBS)

    WB analysis using 60213-1-Ig (same clone as 60213-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60213-1-Ig (transgelin/SM22 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60213-1-PBS in a different storage buffer formulation.

  • WB analysis of human colon using 60213-1-Ig (same clone as 60213-1-PBS)

    WB analysis of human colon using 60213-1-Ig (same clone as 60213-1-PBS)

    human colon tissue were subjected to SDS PAGE followed by western blot with 60213-1-Ig (transgelin/SM22 antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60213-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 60213-1-Ig (same clone as 60213-1-PBS)

    IHC staining of human colon using 60213-1-Ig (same clone as 60213-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60213-1-Ig (transgelin/SM22 antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 60213-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TAGLN antibody included
  • IHC staining of human colon using 60213-1-Ig (same clone as 60213-1-PBS)

    IHC staining of human colon using 60213-1-Ig (same clone as 60213-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60213-1-Ig (transgelin/SM22 antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 60213-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TAGLN antibody included
  • IF Staining of mouse heart using 60213-1-Ig (same clone as 60213-1-PBS)

    IF Staining of mouse heart using 60213-1-Ig (same clone as 60213-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using transgelin/SM22 antibody (60213-1-Ig, Clone: 2A10C2 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60213-1-PBS in a different storage buffer formulation.

  • WB analysis using 60213-1-Ig (same clone as 60213-1-PBS)

    WB analysis using 60213-1-Ig (same clone as 60213-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60213-1-Ig (transgelin/SM22 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60213-1-PBS in a different storage buffer formulation.

transgelin/SM22 Monoclonal antibody, PBS Only

transgelin/SM22 Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 60213-1-PBS

产品说明书

CloneNo. 2A10C2

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF-P, ELISA

别名

SM22, transgelin, 22 kDa actin-binding protein, 2A10C2, Protein WS3-10

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy TAGLN Ready-To-Use IHC Kit. TAGLN primary antibody included.

产品信息

60213-1-PBS targets transgelin/SM22 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 transgelin/SM22 fusion protein Ag0764 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 transgelin
别名 SM22, transgelin, 22 kDa actin-binding protein, 2A10C2, Protein WS3-10
计算分子量 22 kDa
观测分子量 18+22 kDa
GenBank蛋白编号BC004927
基因名称 transgelin/SM22
Gene ID (NCBI) 6876
RRIDAB_11043177
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ01995
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The transgelin family is a group of proteins that belong to 22 kDa actin-related corpnin superfamily. Of all three isoforms, transgelin 1 is the best characterized. Transgelin 1, also known as SM22 alpha, is a specific marker for differentiated smooth muscle cells. Transgelin 2, also known as SM22 beta, is expressed by both smooth muscle and non-smooth muscle cells in a temporally and spatially regulated pattern. Trangenlin 3, also known as NP25, is only found in highly differentiated neuronal cells.