smooth muscle actin specific Monoclonal antibody

smooth muscle actin specific Monoclonal Antibody for FC, IF/ICC,IF-P, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig, rabbit and More (2)

Applications

FC, IF/ICC,IF-P, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1E9A11

Cat No : 67735-1-Ig

Print datasheet

Synonyms

a SMA, AAT6, ACTA2, ACTA2 Smooth muscle Actin, Actin, aortic smooth muscle, ACTSA, ACTVS, Alpha actin 2, Alpha SMA, a-SMA, sma, smooth muscle actin, smooth muscle actin specific, α-sma



经过测试的应用

Positive WB detected inrat stomach tissue, rabbit stomach tissue, pig vein tissue, pig stomach tissue, rabbit large intestine tissue, pig colon tissue, rat colon tissue, mouse colon tissue
Positive IHC detected inhuman colon tissue, human appendicitis tissue, human liver tissue, human liver cancer tissue, rat colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inrat heart tissue, C2C12 cells
Positive FC detected inC2C12 cells
Planning an IHC experiment? We recommend our IHCeasy smooth muscle actin Ready-To-Use IHC Kit. smooth muscle actin primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®594 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67735-1-Ig targets smooth muscle actin specific in WB,IF,FC,IHC,ELISA applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.

Tested Applications FC, IF/ICC,IF-P, IHC, WB, ELISA
Cited ApplicationsWB,IF,IHC
Tested Reactivity Human, mouse, rat, pig, rabbit
Cited Reactivityhuman, mouse, rat, zebrafish, pig
Immunogen Peptide 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name actin, alpha 2, smooth muscle, aorta
Synonyms a SMA, AAT6, ACTA2, ACTA2 Smooth muscle Actin, Actin, aortic smooth muscle, ACTSA, ACTVS, Alpha actin 2, Alpha SMA, a-SMA, sma, smooth muscle actin, smooth muscle actin specific, α-sma
Calculated Molecular Weight 42 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberNM_001613
Gene Symbol ACTA2
Gene ID (NCBI) 59
RRIDAB_2918504
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP62736
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ACTA2 (also known as α-smooth muscle actin or α-SMA) belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. ACTA2 is primarily expressed in vascular smooth muscle and anti-ACTA2 is commonly used to marker smooth muscle cells.

实验方案

Product Specific Protocols
WB protocol for smooth muscle actin specific antibody 67735-1-IgDownload protocol
IHC protocol for smooth muscle actin specific antibody 67735-1-IgDownload protocol
IF protocol for smooth muscle actin specific antibody 67735-1-IgDownload protocol
FC protocol for smooth muscle actin specific antibody 67735-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Adv Mater

A "Bridge-Building" Glycan Scaffold Mimicking Microbial Invasion for In Situ Endothelialization.

Authors - Ruoyu Mu
humanIHC

Mol Cancer

circFARP1 enables cancer-associated fibroblasts to promote gemcitabine resistance in pancreatic cancer via the LIF/STAT3 axis.

Authors - Chonghui Hu
humanIF

Adv Sci (Weinh)

Single-Cell Transcriptomic Analysis of Primary and Metastatic Tumor Ecosystems in Esophageal Squamous Cell Carcinoma

Authors - Yongxu Jia
mouseIF

Acta Pharm Sin B

ADAR1 regulates vascular remodeling in hypoxic pulmonary hypertension through N1-methyladenosine modification of circCDK17

Authors - Junting Zhang
ratWB

Biomaterials

Extended-release of therapeutic microRNA via a host-guest supramolecular hydrogel to locally alleviate renal interstitial fibrosis.

Authors - Yingying Xu
mouseIF

Autophagy

Inhibition of PLA2G4E/cPLA2 promotes survival of random skin flaps by alleviating Lysosomal membrane permeabilization-Induced necroptosis.

Authors - Junsheng Lou