验证数据展示
经过测试的应用
| Positive FC detected in | human PBMCs |
For other applications, we recommend the unconjugated version of this antibody, SF98136-1-PBS
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL647-SF98136 targets scFv Isotype Control in FC applications and shows reactivity with n/a samples.
| 经测试应用 | FC Application Description |
| 经测试反应性 | n/a |
| 免疫原 |
Small molecule 种属同源性预测 |
| 宿主/类型 | Rabbit / scFv |
| 抗体类别 | Recombinant |
| 产品类型 | Single-chain variable fragment antibody (scFv) |
| 全称 | scFv Isotype Control |
| 别名 | Rabbit scFv, Rabbit scFv Isotype, Rabbit scFv Isotype Control |
| 基因名称 | |
| Gene ID (NCBI) | |
| RRID | AB_3748940 |
| 偶联类型 | CoraLite® Plus 647 Fluorescent Dye |
| 最大激发/发射波长 | 654 nm / 674 nm |
| 激发激光 | Red Laser (633 nm) |
| 来源 | Rabbit scFv isotype control from clone 240953C9, consisting of VL-GGGGS linker-VH (N- to C-terminus), with a His tag at the C-terminus. |
| 形式 | Liquid |
| 纯化方式 | Affinity purification |
| 储存缓冲液 | PBS with 4% sucrose and 0.09% sodium azide, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
This product is an isotype control antibody, which is used to estimate the non-specific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 647 scFv Isotype Control antibody CL647-SF98136 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
