• HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human SCF protein

Activity

15-85 ng/mL EC50

Specific Activity

3.6 x 10^5 IU/mg calibrated against SCF reference standard (NIBSC cat no: 91/682). Reference use only. Specific activity is not routinely tested

Species Reactivity

human

Purity

>95%

Cat No : HZ-1024

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Synonyms

c Kit ligand, DKFZp686F2250, KIT ligand, Kitl, KITLG, KL 1, Mast cell growth factor, MGF, SCF, SF, SHEP7, Stem cell factor



Technical Specifications

GeneID 4254
Species Human
Expression HEK293
Activity 15-85 ng/mL EC50
Specific Activity3.6 x 10^5 IU/mg calibrated against SCF reference standard (NIBSC cat no: 91/682). Reference use only. Specific activity is not routinely tested
Purity >95%
Endotoxin<1 EU/µg
Accession NumberP21583
Molecular Mass 35 to 45 kDa, monomer, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human

Stability and Reconstitution

Stability and Storage Lyophilized proteins are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Background

SCF is a hematopoietic growth factor that works by signaling through the c-Kit receptor. SCF and c-Kit are essential for the survival, proliferation, and differentiation of hematopoietic cells (PMID: 12773427)

Synonyms

c Kit ligand, DKFZp686F2250, KIT ligand, Kitl, KITLG, KL 1, Mast cell growth factor, MGF, SCF, SF, SHEP7, Stem cell factor

Publications

SpeciesTitle
human

Blood

LIN28B-mediated expression of fetal hemoglobin and production of fetal-like erythrocytes from adult human erythroblasts ex vivo.

Authors - Y TerryLee
human

J Exp Med

Co-inhibition of NF-κB and JNK is synergistic in TNF-expressing human AML.

Authors - AndrewVolk
human

Mol Cancer Ther

IMGN779, a Novel CD33-Targeting Antibody-Drug Conjugate with DNA Alkylating Activity, Exhibits Potent Antitumor Activity in Models of AML.

Authors - YelenaKovtun
human

Stem Cells

Brief report: efficient generation of hematopoietic precursors and progenitors from human pluripotent stem cell lines.

Authors - Niels-BjarneWoods
human

Oncotarget

Sensitizing leukemia stem cells to NF-κB inhibitor treatment in vivo by inactivation of both TNF and IL-1 signaling.

Authors - JingLi
human

Stem Cell Res Ther

Derivation and propagation of spermatogonial stem cells from human pluripotent cells.

Authors - Huiming Xu