Validation Data Gallery
Technical Specifications
| GeneID | 1437 |
| Species | Human |
| Expression | HEK293 |
| Activity | 0.08-0.8 ng/mL |
| Specific Activity | minimally 6.00x106 IU/mg |
| Purity | >95% |
| Endotoxin | <1 EU/µg |
| Accession Number | P04141 |
| Molecular Mass | 15 to 36 kDa reduced and non-reduced, monomer, glycosylated |
| Formulation | 1x PBS, See Certificate of Analysis for details |
| Species Reactivity | human,mouse,pig |
Stability and Reconstitution
| Stability and Storage | Product Form | Temperature Conditions | Storage Time (From Date of Receipt) |
|---|---|---|---|
| Lyophilized | -20°C to -80°C | Until Expiry Date | |
| Lyophilized | Room Temperature | 2 weeks | |
| Reconstituted as per CofA | -20°C to -80°C | 6 months | |
| Reconstituted as per CofA | 4°C | 1 week | |
| Avoid repeated freeze-thaw cycles. | |||
| Reconstitution | Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1xPBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix. |
Background
Granulocyte/Macrophage Colony-Stimulating Factor (GM-CSF) is a multifunctional cytokine that plays a critical role in hematopoiesis, immune regulation, and cell proliferation. Originally identified for its ability to support the in vitro colony formation of granulocyte-macrophage progenitor cells, GM-CSF also promotes the growth and differentiation of erythroid, megakaryocyte, and eosinophil progenitors. GM-CSF is produced by a wide variety of cell types, including T cells, B cells, macrophages, mast cells, endothelial cells, fibroblasts, and adipocytes, in response to inflammatory signals and cytokine stimulation. Human and murine GM-CSF proteins are species-specific and do not exhibit cross-species reactivity. Recombinant human GM-CSF is a 14.6 kDa globular protein composed of 128 amino acids, featuring two intramolecular disulfide bonds and two potential N-linked glycosylation sites. Due to its important role in immune cell expansion and differentiation, GM-CSF is widely used in immunology, hematopoiesis, cancer research, and cell therapy applications. (PMID 3021817, 2984574, 6390681).
Synonyms
Colony stimulating factor, CSF, CSF2, GM CSF, GMCSF, GM-CSF, Molgramostin, Sargramostim
Publications
| Species | Title |
|---|---|
ACS Nano Engineered Norovirus-Derived Nanoparticles as a Plug-and-Play Cancer Vaccine Platform | |
Blood GM-CSF-dependent pSTAT5 sensitivity is a feature with therapeutic potential in chronic myelomonocytic leukemia. | |
Small Methods Pre-Induced ICD Membrane-Coated Carrier-Free Nanoparticles for the Personalized Lung Cancer Immunotherapy | |
J Exp Med Inherited TNFSF9 deficiency causes broad Epstein-Barr virus infection with EBV+ smooth muscle tumors. | |
ACS Appl Mater Interfaces Combined Chemo-Immuno-Photothermal Therapy for Effective Cancer Treatment via an All-in-One and One-for-All Nanoplatform | |
Biomaterials In situ repurposing of dendritic cells with CRISPR/Cas9-based nanomedicine to induce transplant tolerance. |
