×
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis using 12919-1-AP (same clone as 12919-1-PBS)

    WB analysis using 12919-1-AP (same clone as 12919-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12919-1-AP (PKC Beta antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12919-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 12919-1-AP (same clone as 12919-1-PBS)

    IHC staining of human breast cancer using 12919-1-AP (same clone as 12919-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12919-1-AP (PKC beta antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 12919-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PRKCB antibody included
  • IHC staining of human colon using 12919-1-AP (same clone as 12919-1-PBS)

    IHC staining of human colon using 12919-1-AP (same clone as 12919-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 12919-1-AP (pan-PKC antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12919-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PRKCB antibody included
  • IHC staining of human breast cancer using 12919-1-AP (same clone as 12919-1-PBS)

    IHC staining of human breast cancer using 12919-1-AP (same clone as 12919-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12919-1-AP (PKC beta antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 12919-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PRKCB antibody included
  • FC experiment of K-562 using 12919-1-AP (same clone as 12919-1-PBS)

    FC experiment of K-562 using 12919-1-AP (same clone as 12919-1-PBS)

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human PKC Beta (12919-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 12919-1-PBS in a different storage buffer formulation.

  • IF Staining of zebrafish retina using 12919-1-AP (same clone as 12919-1-PBS)

    IF Staining of zebrafish retina using 12919-1-AP (same clone as 12919-1-PBS)

    Immunofluorescence analysis of bipolar cells labelled with pan-PKC polyclonal antibody (12919-1-AP, green) on tissue sections of the zebrafish retina at 5 days post fertilisation. Dilution 1:300. Tissue fixed overnight in 4% PFA. Müller glia, red; DAPI, blue; HuC/D, magenta. Data generated by Natalia Jaroszynska in Professor Ryan MacDonald's lab, University College London, UK. This data was developed using the same antibody clone with 12919-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse brain using 12919-1-AP (same clone as 12919-1-PBS)

    IP experiment of mouse brain using 12919-1-AP (same clone as 12919-1-PBS)

    IP result of anti-pan-PKC (IP:12919-1-AP, 4ug; Detection:12919-1-AP 1:2000) with mouse brain tissue lysate 2100 ug. This data was developed using the same antibody clone with 12919-1-PBS in a different storage buffer formulation.

  • IF Staining of K-562 using 12919-1-AP (same clone as 12919-1-PBS)

    IF Staining of K-562 using 12919-1-AP (same clone as 12919-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using pan-PKC antibody (12919-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 12919-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

pan-PKC Polyclonal antibody, PBS Only

pan-PKC Polyclonal Antibody for WB, IHC, IF, IF/ICC, FC (Intra), IP, Indirect ELISA
Cat No. 12919-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, zebrafish

应用

WB, IHC, IF, IF/ICC, FC (Intra), IP, Indirect ELISA

别名

PKC beta, PRKCB, EC:2.7.11.13, PKC B, PKCB

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy PRKCB Ready-To-Use IHC Kit. PRKCB primary antibody included.

产品信息

12919-1-PBS targets pan-PKC in WB, IHC, IF, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat, zebrafish samples.

经测试应用 WB, IHC, IF, IF/ICC, FC (Intra), IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, zebrafish
免疫原

CatNo: Ag3591

Product name: Recombinant human PRKCB protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 342-598 aa of BC036472

Sequence: FNFLMVLGKGSFGKVMLSERKGTDELYAVKILKKDVVIQDDDVECTMVEKRVLALPGKPPFLTQLHSCFQTMDRLYFVMEYVNGGDLMYHIQQVGRFKEPHAVFYAAEIAIGLFFLQSKGIIYRDLKLDNVMLDSEGHIKIADFGMCKENIWDGVTTKTFCGTPDYIAPEIIAYQPYGKSVDWWAFGVLLYEMLAGQAPFEGEDEDELFQSIMEHNVAYPKSMSKEAVAICKGLMTKHPGKRLGCGPEGERDIKEHA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protein kinase C, beta
别名 PKC beta, PRKCB, EC:2.7.11.13, PKC B, PKCB
计算分子量 673 aa, 77 kDa
观测分子量 77 kDa
GenBank蛋白编号BC036472
基因名称 PRKCB
Gene ID (NCBI) 5579
RRIDAB_2237469
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP05771
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. Protein kinase Cs (PKCs) are signaling molecules that play key roles in many cellular processes, including secretion, gene expression, proliferation, and muscle contraction. The PKC family is broadly divided into three subgroups: classical, novel, and atypical PKCs. These subgroups differ in both protein sequences and mechanistic requirements for catalytic activity. PKC beta is one of the PKC family members. PKC beta has been reported to be involved in many different cellular functions, such as B cell activation, apoptosis induction, endothelial cell proliferation, and intestinal sugar absorption. Studies in mice also suggest that this kinase may also regulate neuronal functions and correlate fear-induced conflict behavior after stress. This antibody can recognize PKC beta, PKC alpha, PKC gamma, PKC epsilon and PKC delta.

||
New chat

Able™

正在加载，请稍候...