验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, NIH/3T3 cells, HEK-293 cells, HeLa cells, Jurkat cells |
| Positive IHC detected in | mouse testis tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
27809-1-AP targets AKT in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 |
CatNo: Ag26642 Product name: Recombinant human AKT protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 293-405 aa of BC000479 Sequence: FGLCKEGIKDGATMKTFCGTPEYLAPEVLEDNDYGRAVDWWGLGVVMYEMMCGRLPFYNQDHEKLFELILMEEIRFPRTLGPEAKSLLSGLLKKDPKQRLGGGSEDAKEIMQH 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | v-akt murine thymoma viral oncogene homolog 1 |
| 别名 | AKT 1, AKT1, EC:2.7.11.1, PKB, PKB ALPHA |
| 计算分子量 | 56 kDa |
| 观测分子量 | 56-62 kDa |
| GenBank蛋白编号 | BC000479 |
| 基因名称 | AKT1 |
| Gene ID (NCBI) | 207 |
| RRID | AB_3742259 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P31749 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
AKT (also known as protein kinase B, PKB) is a serine threonine protein kinase consisting of three isoforms, AKT1, AKT2, and AKT3, which are encoded by three distinct genes, PKBα, PKBβ, and PKBγ, respectively. Among them, AKT1 is ubiquitously expressed and primarily regulates cell survival, AKT2 controls glucose metabolism, while AKT3 is highly expressed in the brain and testes. These isoforms are central nodes in the PI3K-AKT-mTOR signaling pathway, which is a critical regulator of cell survival, growth, proliferation, and metabolism (PMID: 25811375, PMID: 31173856, PMID: 36180910). This antibody detects total AKT1, AKT2, and AKT3 isoforms, typically showing a molecular weight of approximately 56-62 kDa in Western blot analysis.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for AKT antibody 27809-1-AP | Download protocol |
| WB protocol for AKT antibody 27809-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
