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- KD/KO Validated
NF-κB p65 Polyclonal antibody
NF-κB p65 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (7)
Applications
FC, IF, IHC, IP, WB, ELISA and More (3)
Conjugate
Unconjugated
1169
验证数据展示
经过测试的应用
Positive WB detected in | A431 cells, Hek-293 cells, Jurkat cells, MCF-7 cells, K-562 cells, HeLa cells, Raji cells, NIH/3T3 cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human breast cancer tissue, human stomach tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
Positive FC detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
10745-1-AP targets NF-κB p65 in WB, IP, IHC, IF, FC, CoIP, ChIP, Cell treatment, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | FC, IF, IHC, IP, WB, ELISA |
Cited Applications | Cell treatment, ChIP, CoIP, ELISA, IF, IHC, IP, WB |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, goat, CHICKEN, monkey, rabbit, fish, hamster, pig |
Immunogen | NF-κB p65 fusion protein Ag1199 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | v-rel reticuloendotheliosis viral oncogene homolog A (avian) |
Synonyms | NF Kappa B, NF κB, NFKB 3, NFKB p65, NFKB3, NF-κB, NF-κB3, p65, RELA, Transcription factor p65 |
Calculated Molecular Weight | 65 kDa |
Observed Molecular Weight | 65 kDa |
GenBank Accession Number | BC011603 |
Gene Symbol | RELA |
Gene ID (NCBI) | 5970 |
RRID | AB_2178878 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q04206 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for NF-κB p65 antibody 10745-1-AP | Download protocol |
IHC protocol for NF-κB p65 antibody 10745-1-AP | Download protocol |
IF protocol for NF-κB p65 antibody 10745-1-AP | Download protocol |
IP protocol for NF-κB p65 antibody 10745-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Mol Cell Serine synthesis sustains macrophage IL-1β production via NAD+-dependent protein acetylation | ||
Nat Microbiol IFI16 directly senses viral RNA and enhances RIG-I transcription and activation to restrict influenza virus infection. | ||
Adv Sci (Weinh) OR11H1 Missense Variant Confers the Susceptibility to Vogt-Koyanagi-Harada Disease by Mediating Gadd45g Expression | ||
Sci Adv JLP-centrosome is essential for the microtubule-mediated nucleocytoplasmic transport induced by extracellular stimuli. | ||
Nucleic Acids Res p38-mediated FOXN3 phosphorylation modulates lung inflammation and injury through the NF-κB signaling pathway |