验证数据展示

WB analysis of Hek-293 cells using 10745-1-AP
WB result of RELA, p65antibody (10745-1-AP, 1:500) with si-Control and si-RELA,p65 transfected HeLa cells.
WB analysis using 10745-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10745-1-AP (NF-κB p65 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IHC staining of human breast cancer using 10745-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10745-1-AP (NF-κB p65 antibody) at dilution of 1:200 (under 0x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with NF-κB p65/RELA antibody included
IF Staining of HepG2 using 10745-1-AP
Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 10745-1-AP (p65; RELA antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IP experiment of HeLa using 10745-1-AP
IP result of anti-NF-κB p65 (IP:10745-1-AP, 4ug; Detection:10745-1-AP 1:6000) with HeLa cells lysate 1280 ug.
IHC staining of mouse colon using 10745-1-AP
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 10745-1-AP (NF-κB p65 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with NF-κB p65/RELA antibody included
IHC staining of human colon using 10745-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10745-1-AP (NF-κB p65 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).
Ready-to-use IHC kits available with NF-κB p65/RELA antibody included
IF Staining of HT-1080 using 10745-1-AP
Immunofluorescent analysis of (4% PFA) fixed TNF alpha treated HT-1080 cells using NF-κB p65 antibody (10745-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

Featured Product
KD/KO验证

NF-κB p65 Polyclonal antibody

NF-κB p65 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 10745-1-AP

产品说明书

引用文献(1565)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (7)

应用

WB, IHC, IF/ICC, IP, ELISA and More (3)

别名

NF κB, p65, p65; RELA, RELA, NF Kappa B

缓冲液配方: PBS and Azide

PBS and Azide

偶联物: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite® Plus 647 CoraLite®594

规格:

-/ -

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概述

经过测试的应用
产品信息
发表文章 (1565)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	A431 cells, Jurkat cells, MCF-7 cells, K-562 cells, Hek-293 cells, HeLa cells, Raji cells, NIH/3T3 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human breast cancer tissue, human colon tissue, human liver cancer tissue, human stomach tissue, mouse colon tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells, TNF alpha treated HT-1080 cells

Planning an IHC experiment? We recommend our IHCeasy NF-κB p65/RELA Ready-To-Use IHC Kit. NF-κB p65/RELA primary antibody included.

Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®594 and CoraLite® Plus 647 conjugated versions of this antibody.

应用	推荐稀释比
Western Blot (WB)	WB : 1:1000-1:6000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 23 publications below
WB	See 1412 publications below
IHC	See 121 publications below
IF	See 243 publications below
IP	See 10 publications below
ELISA	See 1 publications below
CoIP	See 8 publications below
ChIP	See 9 publications below

产品信息

10745-1-AP targets NF-κB p65 in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用	WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用	WB, IHC, IF, IP, CoIP, chIP, ELISA
经测试反应性	human, mouse, rat
文献引用反应性	human, mouse, rat, rabbit, monkey, chicken, hamster, sheep, goat, fish
免疫原	NF-κB p65 fusion protein Ag1199 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	v-rel reticuloendotheliosis viral oncogene homolog A (avian)
别名	NF κB, p65, p65; RELA, RELA, NF Kappa B
计算分子量	65 kDa
观测分子量	65 kDa
GenBank蛋白编号	BC011603
基因名称	NF-κB p65
Gene ID (NCBI)	5970
RRID	AB_2178878
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	Q04206
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

Nuclear factor k B (NF-kB) is a sequence-specific DNA-binding protein complex which regulates the expression of viral genomes, including the human immunodeficiency virus, and a variety of cellular genes, particularly those involved in immune and inflammatory responses. The members of the NF-kB family in mammalian cells include the proto-oncogene c-Rel,p50/p105 (NFkB1), p65 (RelA), p52/p100 (NFkB2), and RelB. All of these proteins share a conserved 300-amino acid region known as the Rel homology domain which is responsible for DNA binding, dimerization, and nuclear translocation of NF-kB. The p65 subunit is a major component of NF-kB complexes and is responsible for trans-activation. NF-kB heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kB p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of IkB upon NF-kB the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kB complex. It associates with chromatin at the NF-kB promoter region via association with DDX1. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human RELA.

实验方案

Product Specific Protocols
WB protocol for NF-κB p65 antibody 10745-1-AP	Download protocol
IHC protocol for NF-κB p65 antibody 10745-1-AP	Download protocol
IF protocol for NF-κB p65 antibody 10745-1-AP	Download protocol
IP protocol for NF-κB p65 antibody 10745-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
mouse	WB	Bioact Mater Silicate ions as soluble form of bioactive ceramics alleviate aortic aneurysm and dissection Authors - Yumei Que View Article
mouse	IF	Blood PMID: 32291445 Authors - Kunpeng Wu View Article
mouse	WB	Mol Cell Serine synthesis sustains macrophage IL-1β production via NAD+-dependent protein acetylation Authors - Chuanlong Wang View Article
human	WB	Nat Microbiol IFI16 directly senses viral RNA and enhances RIG-I transcription and activation to restrict influenza virus infection. Authors - Zhimin Jiang View Article
human	WB	Adv Sci (Weinh) OR11H1 Missense Variant Confers the Susceptibility to Vogt-Koyanagi-Harada Disease by Mediating Gadd45g Expression Authors - Xingran Li View Article
mouse	WB	Adv Sci (Weinh) Sirtuin 5-Mediated Desuccinylation of ALDH2 Alleviates Mitochondrial Oxidative Stress Following Acetaminophen-Induced Acute Liver Injury Authors - Qiwen Yu View Article

验证数据展示

WB analysis of Hek-293 cells using 10745-1-AP

WB analysis using 10745-1-AP

IHC staining of human breast cancer using 10745-1-AP

IF Staining of HepG2 using 10745-1-AP

IP experiment of HeLa using 10745-1-AP

IHC staining of mouse colon using 10745-1-AP

IHC staining of human colon using 10745-1-AP

IF Staining of HT-1080 using 10745-1-AP

NF-κB p65 Polyclonal antibody

NF-κB p65 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Silicate ions as soluble form of bioactive ceramics alleviate aortic aneurysm and dissection

PMID: 32291445

Serine synthesis sustains macrophage IL-1β production via NAD+-dependent protein acetylation

IFI16 directly senses viral RNA and enhances RIG-I transcription and activation to restrict influenza virus infection.

OR11H1 Missense Variant Confers the Susceptibility to Vogt-Koyanagi-Harada Disease by Mediating Gadd45g Expression

Sirtuin 5-Mediated Desuccinylation of ALDH2 Alleviates Mitochondrial Oxidative Stress Following Acetaminophen-Induced Acute Liver Injury