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验证数据展示

  • WB analysis using RMX00006

    WB analysis using RMX00006

    Various lysates were subjected to SDS PAGE followed by western blot with RMX00006 (p38 MAPK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 using RMX00006

    IP experiment of HEK-293 using RMX00006

    IP result of anti-p38 MAPK (IP:RMX00006, 4ug; Detection:RMX00006 1:10000) with HEK-293 cells lysate 1360 ug.

  • IHC staining of mouse heart using RMX00006

    IHC staining of mouse heart using RMX00006

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using RMX00006 (p38 MAPK antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with p38 MAPK antibody included
  • IHC staining of mouse heart using RMX00006

    IHC staining of mouse heart using RMX00006

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using RMX00006 (p38 MAPK antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with p38 MAPK antibody included
  • IHC staining of human lung cancer using RMX00006

    IHC staining of human lung cancer using RMX00006

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using RMX00006 (p38 MAPK antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with p38 MAPK antibody included
  • IHC staining of human lung cancer using RMX00006

    IHC staining of human lung cancer using RMX00006

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using RMX00006 (p38 MAPK antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with p38 MAPK antibody included
  • IF Staining of HepG2 using RMX00006

    IF Staining of HepG2 using RMX00006

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using p38 MAPK antibody (RMX00006) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

Multi-rAb™ p38 MAPK Multi-Recombinant antibody

p38 MAPK Multi-Recombinant Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. RMX00006

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IP, ELISA

别名

MAPK14, Cytokine suppressive anti-inflammatory drug-binding protein, CSPB1, CSBP2, CSBP1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, Jurkat cells, NIH/3T3 cells, PC-12 cells, mouse heart tissue, rat heart tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman lung cancer tissue, mouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy p38 MAPK Ready-To-Use IHC Kit. p38 MAPK primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

RMX00006 targets p38 MAPK in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Multi-Recombinant
产品类型 Antibody
全称 mitogen-activated protein kinase 14
别名 MAPK14, Cytokine suppressive anti-inflammatory drug-binding protein, CSPB1, CSBP2, CSBP1
计算分子量 360 aa, 41 kDa
观测分子量38-42 kDa
GenBank蛋白编号BC031574
基因名称 p38 MAPK
Gene ID (NCBI) 1432
偶联类型 Unconjugated
形式 Liquid
纯化方式N/A
UNIPROT IDQ16539
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MAPK14(mitogen-activated protein kinase 14) is also named as SAPK2A, p38MAPK, CSBP1, RK, p38, EXIP, Mxi2, CSBP2, PRKM14, PRKM15, CSPB1, p38ALPHA and belongs to the MAP kinase subfamily. MAPK14-signaling is a central pathway for the integration of instructive signals in dendritic cells for T(H)17 differentiation and inflammation(PMID:22231518). It plays an important role in the regulation of hematopoietic stem cell self-renewal in vitro and inhibition of MAPK14 activation with a small molecule inhibitor may represent a novel approach to promote ex vivo expansion of hematopoietic stem cell(PMID:21198398). This protein has 4 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for p38 MAPK antibody RMX00006Download protocol
IHC protocol for p38 MAPK antibody RMX00006Download protocol
IF protocol for p38 MAPK antibody RMX00006Download protocol
IP protocol for p38 MAPK antibody RMX00006Download protocol
Standard Protocols
Click here to view our Standard Protocols