Various lysates were subjected to SDS PAGE followed by western blot with 80821-3-RR (p38 MAPK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80821-3-PBS in a different storage buffer formulation.

mouse brain tissue were subjected to SDS PAGE followed by western blot with 80821-3-RR (p38 MAPK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80821-3-PBS in a different storage buffer formulation.

WB result of p38 MAPK antibody (80821-3-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-p38 MAPK transfected HepG2 cells. This data was developed using the same antibody clone with 80821-3-PBS in a different storage buffer formulation.

IP result of anti-p38 MAPK (IP:80821-3-RR, 4ug; Detection:80821-3-RR 1:2000) with HEK-293 cells lysate 1575 ug. This data was developed using the same antibody clone with 80821-3-PBS in a different storage buffer formulation.

1x10^6 HeLa cells were intracellularly stained with 0.25 ug Anti-Human p38 MAPK (80821-3-RR, Clone:230263H7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 80821-3-PBS in a different storage buffer formulation.

1x10^6 Jurkat cells were intracellularly stained with 0.25 ug Anti-Human p38 MAPK (80821-3-RR, Clone:230263H7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 80821-3-PBS in a different storage buffer formulation.

Biolayer interferometry (BLl) kinetic assays of 80821-3-RR against Human p38 MAPK were performed. The affinity constant is 1.04 nM.