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验证数据展示

  • WB analysis using 68100-1-Ig (same clone as 68100-1-PBS)

    WB analysis using 68100-1-Ig (same clone as 68100-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68100-1-Ig (p115, USO1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Lamin B1 Monoclonal antibody (66095-1-Ig) as loading control. This data was developed using the same antibody clone with 68100-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 68100-1-Ig (same clone as 68100-1-PBS)

    IHC staining of human liver cancer using 68100-1-Ig (same clone as 68100-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68100-1-Ig (p115, USO1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68100-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with USO1 antibody included
  • IHC staining of human liver cancer using 68100-1-Ig (same clone as 68100-1-PBS)

    IHC staining of human liver cancer using 68100-1-Ig (same clone as 68100-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68100-1-Ig (p115, USO1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68100-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with USO1 antibody included
  • IF Staining of HeLa using 68100-1-Ig (same clone as 68100-1-PBS)

    IF Staining of HeLa using 68100-1-Ig (same clone as 68100-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using p115, USO1 antibody (68100-1-Ig, Clone: 3B7D8 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68100-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 68100-1-Ig (same clone as 68100-1-PBS)

    WB analysis of HeLa using 68100-1-Ig (same clone as 68100-1-PBS)

    WB result of p115, USO1 antibody (68100-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-p115, USO1 transfected HeLa cells. This data was developed using the same antibody clone with 68100-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 68100-1-Ig (same clone as 68100-1-PBS)

    IF Staining of HeLa using 68100-1-Ig (same clone as 68100-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using p115, USO1 antibody (68100-1-Ig, Clone: 3B7D8 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68100-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

p115, USO1 Monoclonal antibody, PBS Only

p115, USO1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 68100-1-PBS

产品说明书

CloneNo. 3B7D8

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

USO1, 3B7D8, General vesicular transport factor p115, Protein USO1 homolog, TAP

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy USO1 Ready-To-Use IHC Kit. USO1 primary antibody included.

产品信息

68100-1-PBS targets p115, USO1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag5543

Product name: Recombinant human p115, USO1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 660-961 aa of BC032654

Sequence: EQDLQLEELRQQVSTLKCQNEQLQTAVTQQVSQIQQHKDQYNLLKIQLGKDNQHQGSYSEGAQMNGIQPEEIGRLREEIEELKRNQELLQSQLTEKDSMIENMKSSQTSGTNEQSSAIVSARDSEQVAELKQELATLKSQLNSQSVEITKLQTEKQELLQKTEAFAKSVEVQGETETIIATKTTDVEGRLSALLQETKELKNEIKALSEERTAIKEQLDSSNSTIAILQTEKDKLELEITDSKKEQDDLLVLLADQDQKILSLKNKLKDLGHPVEEEDELESGDQEDEDDESEDPGKDLDHI

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 USO1 homolog, vesicle docking protein (yeast)
别名 USO1, 3B7D8, General vesicular transport factor p115, Protein USO1 homolog, TAP
计算分子量 962 aa, 108 kDa
观测分子量108 kDa
GenBank蛋白编号BC032654
基因名称 USO1
Gene ID (NCBI) 8615
RRIDAB_2918835
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO60763
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

p115, also known as USO1, TAP (transcytosis-associated protein) or VDP (vesicle docking protein) is a general vesicular transport factor and plays an important role at different steps of vesicular transport. It is a 962-residue peripheral membrane protein which recycles between the cytosol and the Golgi apparatus during interphase (PMID: 9478999). p115 forms stable homodimers (PMID: 19247479). Rab1 recruits p115 to coat protein complex II (COPII) vesicles during budding from the endoplasmic reticulum, where p115 interacts directly with a select set of SNARE proteins (PMID: 10903204). p115 is required for intra-Golgi transport, and also functions in endoplasmic reticulum to Golgi trafficking, Golgi biogenesis and exocytotic transport (PMID: 19247479).

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