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Recombinant Mouse IL-1 alpha protein (His Tag)(HPLC verified)

种属

Mouse

纯度

>90 %, SDS-PAGE
>90 %, SEC-HPLC

标签

His Tag

生物活性

未测试

Cat no : Eg0263

Print datasheet

Synonyms

IL1a, IL1-alpha, Il 1a, IL1 alpha, IL-1,IL1

验证数据展示

  • Purity of Mouse IL-1 alpha was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Mouse IL-1 alpha was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

  • The purity of Mouse IL-1 alpha was greater than 90% as determined by SEC-HPLC.

    The purity of Mouse IL-1 alpha was greater than 90% as determined by SEC-HPLC.

产品信息

纯度 >90 %, SDS-PAGE
>90 %, SEC-HPLC
内毒素 <0.1 EU/μg protein, LAL method
生物活性 
Not tested
来源 HEK293-derived Mouse IL-1 alpha protein Met+Ser115-Ser270 (Accession# P01582) with a His tag at the C-terminus.
基因ID 16175
蛋白编号 P01582
预测分子量 21.9 kDa
SDS-PAGE 24-32 kDa, reducing (R) conditions
组分 Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
复溶 Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
储存条件
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
运输条件 The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

背景信息

Interleukin-1α (Il-1α) is a protein that plays a crucial role in the regulation of a number of key cellular processes. Il-1α is a cytokine and a potent mediator of body's response to inflammation, microbial invasion, tissue injury and immunological response. In addition, recent studies suggest that Il-1α also has a role in wound healing, rheumatoid arthritis, Alzheimer's disease and tumor growth. Il-1α functions as an alarmin during the process of sterile inflammation. It serves as a signal from dying cells to potentiate the inflammatory response, increasing the expression of other secreted factors like IL-6 and IL-8. Il-1α was found to affect the cell cycle of osteosarcoma cell to reduce cell growth.

参考文献:

1. Dinarello CA. et al. (2009). Annu Rev Immunol. 27:519-50.
2. Dinarello CA. et al. (1998). Int Rev Immunol. 16:457-99.
3. Infante J. et al. (2007). Dement Geriatr Cogn Disord. 23:215-8.
4. Apte RN. et al. (2008). Immunol Rev. 222:222-41.
5. Palmer G. et al. (2005). Cytokine. 2:153-60.
6. Rider P. et al. (2013). Semin Immunol. 25(6):430-8.
7. Orjalo AV. et al. (2009). Proc Natl Acad Sci U S A. 106(40):17031-6.