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  • Featured Product
  • KD/KO Validated

mTOR Monoclonal antibody, PBS Only

mTOR Monoclonal Antibody for WB,IP,Indirect ELISA,IHC,IF

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat

Applications

WB,IP,Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

1G11A3

Cat No : 66888-1-PBS

Print datasheet

Synonyms

FLJ44809, FRAP, FRAP1, FRAP2, Mammalian target of rapamycin, MTOR, RAFT1, Rapamycin target protein 1, RAPT1

验证数据展示

  • WB analysis using 66888-1-Ig (same clone as 66888-1-PBS)

    WB analysis using 66888-1-Ig (same clone as 66888-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66888-1-Ig (mTOR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66888-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66888-1-Ig (same clone as 66888-1-PBS)

    IF Staining of HepG2 using 66888-1-Ig (same clone as 66888-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using mTOR antibody (66888-1-Ig, Clone: 1G11A3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66888-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 66888-1-Ig (same clone as 66888-1-PBS)

    WB analysis of HeLa using 66888-1-Ig (same clone as 66888-1-PBS)

    WB result of mTOR antibody (66888-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-mTOR transfected HeLa cells. This data was developed using the same antibody clone with 66888-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 66888-1-Ig (same clone as 66888-1-PBS)

    WB analysis of HeLa using 66888-1-Ig (same clone as 66888-1-PBS)

    Non-treated and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 67778-1-Ig (Phospho-mTOR (Ser2448) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with RICTOR antibody (66867-2-Ig) and mTOR antibody (66888-1-Ig) subsequently. This data was developed using the same antibody clone with 66888-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 66888-1-Ig (same clone as 66888-1-PBS)

    WB analysis of HEK-293 using 66888-1-Ig (same clone as 66888-1-PBS)

    Non-treated and Rapamycin treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 67778-1-Ig (Phospho-mTOR (Ser2448) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with RICTOR antibody (66867-2-Ig) and mTOR antibody (66888-1-Ig) subsequently. This data was developed using the same antibody clone with 66888-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 66888-1-Ig (same clone as 66888-1-PBS)

    WB analysis of HEK-293 using 66888-1-Ig (same clone as 66888-1-PBS)

    Non-treated and Calyculin A treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 67778-1-Ig (Phospho-mTOR (Ser2448) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with RICTOR antibody (66867-2-Ig) and mTOR antibody (66888-1-Ig) subsequently. This data was developed using the same antibody clone with 66888-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 66888-1-Ig (same clone as 66888-1-PBS)

    IHC staining of human colon cancer using 66888-1-Ig (same clone as 66888-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66888-1-Ig (MTOR antibody) at dilution of 1:2500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66888-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 66888-1-Ig (same clone as 66888-1-PBS)

    IHC staining of human colon cancer using 66888-1-Ig (same clone as 66888-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66888-1-Ig (MTOR antibody) at dilution of 1:2500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66888-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 66888-1-Ig (same clone as 66888-1-PBS)

    IP experiment of HeLa using 66888-1-Ig (same clone as 66888-1-PBS)

    IP result of anti-mTOR (IP:66888-1-Ig, 5ug; Detection:66888-1-Ig 1:20000) with HeLa cells lysate 1600 ug. This data was developed using the same antibody clone with 66888-1-PBS in a different storage buffer formulation.

产品信息

66888-1-PBS targets mTOR in WB, IP, IF, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB,IP,Indirect ELISA,IHC,IF
Tested Reactivity Human, Mouse, Rat
Immunogen mTOR fusion protein Ag28395 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name FK506 binding protein 12-rapamycin associated protein 1
Synonyms FLJ44809, FRAP, FRAP1, FRAP2, Mammalian target of rapamycin, MTOR, RAFT1, Rapamycin target protein 1, RAPT1
Calculated Molecular Weight 289 kDa
Observed Molecular Weight 250-289 kDa
GenBank Accession NumberNM_004958
Gene Symbol FRAP1
Gene ID (NCBI) 2475
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP42345
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

MTOR, also named as FRAP1, FRAP, FRAP2 and RAPT1, belongs to the PI3/PI4-kinase family. MTOR is a Ser/Thr protein kinase that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth. MTOR is kinase subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. MTOR has a calculated molecular mass of 289 kDa, and always can be detected at about 250 kDa due to some modifications (PMID: 14578359).