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验证数据展示

  • WB analysis using 81202-2-RR

    WB analysis using 81202-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81202-2-RR (mCherry antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • FC experiment of Transfected HEK-293T using 81202-2-RR

    FC experiment of Transfected HEK-293T using 81202-2-RR

    1x10^6 HEK-293T cells or mCherry Transfected HEK-293T cells were intracellularly stained with 0.25 ug mCherry Recombinant antibody (81202-2-RR, Clone:241830F8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of Transfected HEK-293 using 81202-2-RR

    IF Staining of Transfected HEK-293 using 81202-2-RR

    Immunofluorescent analysis of (4% PFA) fixed Transfected HEK-293 cells using mCherry antibody (81202-2-RR, Clone: 241830F8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 81202-2-RR

    Affinity and Kinetic Characterization of 81202-2-RR

    Biolayer interferometry (BLl) kinetic assays of 81202-2-RR against mCherry were performed. The affinity constant is 0.205 nM.

mCherry Recombinant antibody

mCherry Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 81202-2-RR

产品说明书

CloneNo. 241830F8

宿主/亚型

Rabbit / IgG

种属反应性

recombinant protein

应用

WB, IF/ICC, FC (Intra), ELISA

别名

241830F8

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inTransfected HEK-293T cells
Positive IF/ICC detected inTransfected HEK-293 cells
Positive FC (Intra) detected inTransfected HEK-293T cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

81202-2-RR targets mCherry in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with recombinant protein samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 recombinant protein
免疫原 mCherry fusion protein Ag25320 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 mCherry
别名 241830F8
计算分子量 27 kDa
基因名称
Gene ID (NCBI)
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Red fluorescent proteins (RFPs) is a collective term referring to a heterogeneous group of red chromophore-carrying proteins, originating from various species and forming different protein lineages. The original RFP (dsRed) is a 225 amino acid fluorescent protein (25.9 kDa) derived from Discosoma sp.. It emits red light with a peak wavelength of 593 nm upon excitation by green light (excitation peak at 558 nm). When fused with other proteins, RFP serves as a versatile reporter protein e.g. for quantifying expression levels or facilitating visualization of subcellular localization through fluorescence microscopy. This antibody is a rabbit polyclonal antibody raised against mCherry.

实验方案

Product Specific Protocols
WB protocol for mCherry antibody 81202-2-RRDownload protocol
IF protocol for mCherry antibody 81202-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols