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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis using 26765-1-AP (same clone as 26765-1-PBS)

    WB analysis using 26765-1-AP (same clone as 26765-1-PBS)

    mCherry tagged protein was subjected to SDS PAGE followed by western blot with 26765-1-AP (mCherry antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26765-1-PBS in a different storage buffer formulation.

  • WB analysis of Transfected HEK-293 using 26765-1-AP (same clone as 26765-1-PBS)

    WB analysis of Transfected HEK-293 using 26765-1-AP (same clone as 26765-1-PBS)

    WB result of mCherry antibody (26765-1-AP; 1:2000; room temperature for 1.5 hours) with negative control and mCherry overexpressed Transfected HEK-293 cells. This data was developed using the same antibody clone with 26765-1-PBS in a different storage buffer formulation.

  • IP experiment of Transfected HEK-293T using 26765-1-AP (same clone as 26765-1-PBS)

    IP experiment of Transfected HEK-293T using 26765-1-AP (same clone as 26765-1-PBS)

    IP result of anti-mCherry (IP:26765-1-AP, 4ug; Detection:26765-1-AP 1:2000) with Transfected HEK-293T cells lysate 1280 ug. This data was developed using the same antibody clone with 26765-1-PBS in a different storage buffer formulation.

  • IF Staining of Transfected HEK-293 using 26765-1-AP (same clone as 26765-1-PBS)

    IF Staining of Transfected HEK-293 using 26765-1-AP (same clone as 26765-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using mCherry antibody (26765-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 26765-1-PBS in a different storage buffer formulation.

mCherry Polyclonal antibody, PBS Only

mCherry Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 26765-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

recombinant protein

应用

WB, IF/ICC, IP, Indirect ELISA

别名

Red fluorescence protein, red fluorescent protein, RFP, RFP-tag

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

26765-1-PBS targets mCherry in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with recombinant protein samples.

经测试应用 WB, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 recombinant protein
免疫原

CatNo: Ag25320

Product name: Recombinant mCherry protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-235 aa of

Sequence: VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAEGRHSTGGMDELYK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mCherry
别名 Red fluorescence protein, red fluorescent protein, RFP, RFP-tag
计算分子量 27 kDa
基因名称
Gene ID (NCBI)
RRIDAB_2876881
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Red fluorescent proteins (RFPs) is a collective term referring to a heterogenous group of red chromophore-carrying proteins, originating from various species and forming different protein lineages.

The original RFP (dsRed) is a 225 amino acid fluorescent protein (25.9 kDa) derived from Discosoma sp.. It emits red light with a peak wavelength of 593 nm upon excitation by green light (excitation peak at 558 nm).

When fused with other proteins, RFP serves as a versatile reporter protein e.g. for quantifying expression levels or facilitates visualization of subcellular localization through fluorescence microscopy.

This antibody is a rabbit polyclonal antibody raised against mCherry. It can be used to detect mCherry, dsRed, tdTomato, and mScarlet.