验证数据展示
经过测试的应用
| Positive WB detected in | RAW 264.7 cells, LPS treated human PBMCs |
| Positive IHC detected in | mouse brain tissue, human colon tissue, rat brain tissue, human liver tissue, human lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | RAW 264.7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
80517-1-RR targets iNOS in WB, IHC, IF/ICC, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, ChIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | nitric oxide synthase 2, inducible |
| 别名 | NOS2, 6O22, EC:1.14.13.39, Hepatocyte NOS, HEP-NOS |
| 计算分子量 | 131 kDa |
| 观测分子量 | 110-130 kDa |
| GenBank蛋白编号 | NM_000625 |
| 基因名称 | iNOS |
| Gene ID (NCBI) | 4843 |
| RRID | AB_2918898 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P35228 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NOS2, also named as iNOS and NOS2A, produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. NO is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission, antimicrobial and antitumoral activities. NOS2 is a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. iNOS has a very short half-life due to rapid degradation by calpain. iNOS monomer is a direct substrate of calpain I and can be cleaved by calpain I at the canonical CaM-binding site(503-532aa) of iNOS, and then a ~70kD band can be detected by western (PMID:11786228). This antibody is specific to NOS2.
发表文章
| Species | Application | Title |
|---|---|---|
Bioact Mater Bioengineered extracellular vesicles escape lysosomal degradation and deliver Tet-PKM2 for macrophage immunometabolic reprogramming and periodontitis treatment. | ||
Nat Commun Acid-resistant chemotactic DNA micromotors for probiotic delivery in inflammatory bowel disease | ||
Nat Commun Porous microneedle patch with sustained delivery of extracellular vesicles mitigates severe spinal cord injury | ||
Nat Commun Inhalable nanovesicles loaded with a STING agonist enhance CAR-T cell activity against solid tumors in the lung | ||
Nat Commun Metabolomics and proteomics reveal blocking argininosuccinate synthetase 1 alleviates colitis in mice | ||
ACS Nano Biotin-Avidin System-Based Delivery Enhances the Therapeutic Performance of MSC-Derived Exosomes |
