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验证数据展示

  • WB analysis of human plasma using 67538-1-Ig (same clone as 67538-1-PBS)

    WB analysis of human plasma using 67538-1-Ig (same clone as 67538-1-PBS)

    human plasma (0.1 ul) were subjected to SDS PAGE followed by western blot with 67538-1-Ig (human IgD antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67538-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 67538-1-Ig (same clone as 67538-1-PBS)

    IHC staining of human tonsillitis using 67538-1-Ig (same clone as 67538-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67538-1-Ig (human IgD antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67538-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with human IgD antibody included
  • IHC staining of human tonsillitis using 67538-1-Ig (same clone as 67538-1-PBS)

    IHC staining of human tonsillitis using 67538-1-Ig (same clone as 67538-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67538-1-Ig (human IgD antibody) at dilution of 1:20000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67538-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with human IgD antibody included
  • IHC staining of human tonsillitis using 67538-1-Ig (same clone as 67538-1-PBS)

    IHC staining of human tonsillitis using 67538-1-Ig (same clone as 67538-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67538-1-Ig (human IgD antibody) at dilution of 1:20000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67538-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with human IgD antibody included
  • IF Staining of human tonsillitis using 67538-1-Ig (same clone as 67538-1-PBS)

    IF Staining of human tonsillitis using 67538-1-Ig (same clone as 67538-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using IGHD antibody (67538-1-Ig, Clone: 1D1B12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67538-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 67538-1-Ig (same clone as 67538-1-PBS)

    IHC staining of human tonsillitis using 67538-1-Ig (same clone as 67538-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67538-1-Ig (human IgD antibody) at dilution of 1:40000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67538-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with human IgD antibody included

human IgD Monoclonal antibody, PBS Only

human IgD Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 67538-1-PBS

产品说明书

CloneNo. 1D1B12

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

WB, IHC, IF-P, Indirect ELISA

别名

IGHD, IgD, 1D1B12

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy human IgD Ready-To-Use IHC Kit. human IgD primary antibody included.

产品信息

67538-1-PBS targets human IgD in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag9614

Product name: Recombinant human IGHD protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 202-548 aa of BC021276

Sequence: RRDSYYMTSSQLSTPLQQWRQGEYKCVVQHTASKSKKEIFRWPESPKAQASSVPTAQPQAEGSLAKATTAPATTRNTGRGGEEKKKEKEKEEQEERETKTPECPSHTQPLGVYLLTPAVQDLWLRDKATFTCFVVGSDLKDAHLTWEVAGKVPTGGVEEGLLERHSNGSQSQHSRLTLPRSLWNAGTSVTCTLNHPSLPPQRLMALREPAAQAPVKLSLNLLASSDPPEAASWLLCEVSGFSPPNILLMWLEDQREVNTSGFAPARPPPQPGSTTFWAWSVLRVPAPPSPQPATYTCVVSHEDSRTLLNASRSLEVSYLAMTPLIPQSKDENSDDYTTFDDVGSLWT

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 immunoglobulin heavy constant delta
别名 IGHD, IgD, 1D1B12
计算分子量 573 aa, 63 kDa
观测分子量 55-70 kDa
GenBank蛋白编号BC021276
基因名称 IGHD
Gene ID (NCBI) 3495
RRIDAB_2882757
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

IgD is the major antigen receptor isotype on the surface of most peripheral B-cells. The function of IgD is to signal the B cells to be activated. The relative molecular mass and half-life of secreted IgD is 185 kDa and 2.8 days, respectively. Secreted IgD is produced as a monomeric antibody with two heavy chains of the delta (δ) class, and two Ig light chains. This antibody detects the heavy chain (55-70 kDa) of IgD and secreted IgD (185 kDa) .