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验证数据展示

  • WB analysis using 33278-1-AP

    WB analysis using 33278-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 33278-1-AP (hCG Beta antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. Negative control: human heart tissue, human kidney tissue.

  • IHC staining of human placenta using 33278-1-AP

    IHC staining of human placenta using 33278-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 33278-1-AP (hCG Beta antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human placenta using 33278-1-AP

    IHC staining of human placenta using 33278-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 33278-1-AP (hCG Beta antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

hCG Beta Polyclonal antibody

hCG Beta Polyclonal Antibody for WB, IHC, ELISA
Cat No. 33278-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

CGB5, hCG, hCGB

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inJAR cells, Forskolin treated JAR cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

33278-1-AP targets hCG Beta in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag38044

Product name: Recombinant human CGB5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 21-165 aa of BC051378

Sequence: SKEPLRPRCRPINATLAVEKEGCPVCITVNTTICAGYCPTMTRVLQGVLPALPQVVCNYRDVRFESIRLPGCPRGVNPVVSYAVALSCQCALCRRSTTDCGGPKDHPLTCDDPRFQDSSSSKAPPPSLPSPSRLPGPSDTPILPQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chorionic gonadotropin, beta polypeptide 5
别名 CGB5, hCG, hCGB
计算分子量 165 aa, 18 kDa
观测分子量 19, 21 kDa
GenBank蛋白编号BC051378
基因名称 CGB5
Gene ID (NCBI) 93659
RRIDAB_3742923
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDP0DN86
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Beta-hCG (beta-human chorionic gonadotropin) is a glycoprotein hormone produced by the placenta during pregnancy. It is a critical marker used in both obstetrics and oncology for various diagnostic and monitoring purposes. hCG is composed of two subunits: an alpha subunit (similar to other glycoprotein hormones like LH, FSH, and TSH) and a unique beta subunit (β-hCG). The beta subunit is the primary focus for diagnostic tests due to its specificity. β-hCG is initially secreted by the syncytiotrophoblast cells surrounding the developing embryo and later by the placenta.

实验方案

Product Specific Protocols
IHC protocol for hCG Beta antibody 33278-1-APDownload protocol
WB protocol for hCG Beta antibody 33278-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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