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验证数据展示

  • WB analysis using 85800-9-RR (same clone as 85800-9-PBS)

    WB analysis using 85800-9-RR (same clone as 85800-9-PBS)

    Hydroxyurea treated JAR cells were subjected to SDS PAGE followed by western blot with 85800-9-RR (CGA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85800-9-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 85800-9-RR (same clone as 85800-9-PBS)

    IHC staining of human placenta using 85800-9-RR (same clone as 85800-9-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 85800-9-RR (CGA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85800-9-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 85800-9-RR (same clone as 85800-9-PBS)

    IHC staining of human placenta using 85800-9-RR (same clone as 85800-9-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 85800-9-RR (CGA antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85800-9-PBS in a different storage buffer formulation.

  • IF Staining of JAR using 85800-9-RR (same clone as 85800-9-PBS)

    IF Staining of JAR using 85800-9-RR (same clone as 85800-9-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Hydroxyurea treated JAR cells using hCG Alpha antibody (85800-9-RR, Clone: 250136C4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85800-9-PBS in a different storage buffer formulation.

hCG Alpha Recombinant monoclonal antibody, PBS Only

hCG Alpha Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 85800-9-PBS

产品说明书

CloneNo. 250136C4

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

CGA, HCG, Anterior pituitary glycoprotein hormones common subunit alpha, CG ALPHA, CG-alpha

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

85800-9-PBS targets hCG Alpha in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Eg3377

Product name: Recombinant Human hCG Alpha protein (rFc Tag) (HPLC verified)

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 25-116 aa of

Sequence: APDVQDCPECTLQENPFFSQPGAPILQCMGCCFSRAYPTPLRSKKTMLVQKNVTSESTCCVAKSYNRVTVMGGFKVENHTACHCSTCYYHKS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 hCG Alpha
别名 CGA, HCG, Anterior pituitary glycoprotein hormones common subunit alpha, CG ALPHA, CG-alpha
计算分子量18 kDa
观测分子量20 kDa
基因名称
Gene ID (NCBI) 1082
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP0DN86(CGB)+P01215(CGA)
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Human chorionic gonadotropin (hCG) is a heterodimeric glycoprotein hormone consisting of two non-covalently linked subunits: a common α-subunit (hCGα) and a hormone-specific β-subunit (hCGβ). The α-subunit, also designated CGA (chorionic gonadotropin alpha chain), is a 92-amino-acid glycopeptide of ~14-22 kDa that is identical to the α-subunits of the pituitary glycoprotein hormones luteinizing hormone (LH), follicle-stimulating hormone (FSH), and thyroid-stimulating hormone (TSH). Because the α-subunit is shared among these hormones, its biological specificity is conferred by the unique β-subunit with which it associates.

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