验证数据展示
经过测试的应用
| Positive WB detected in | Recombinant protein |
| Positive IF/ICC detected in | Transfected HEK-293 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10000-0-AP targets GST Tag in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with recombinant protein samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, CoIP, ChIP, ELISA |
| 经测试反应性 | recombinant protein |
| 文献引用反应性 | human |
| 免疫原 |
Recombinant protein 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | GST Tag |
| 别名 | Glutathione S-Transferase, GST |
| 计算分子量 | 26 kDa |
| GenBank蛋白编号 | FN315687 |
| 基因名称 | |
| Gene ID (NCBI) | |
| RRID | AB_11042316 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GST(Glutathione S-Transferase) is a widely used protein tag encoded by the Schistosoma japonicum. GST provides both an easily detectable tag and a simple purification process with little effect on the biological function of the protein of interest. This GST-fusion protein can then be purified from cells via its high affinity for glutathione. Antibodies to GST are useful for checking protein expression both in plaques and on western blots as well as for immunoaffinity purification of proteins expressed in cells. This antibody can be used to detect the GST tagged protein. This antibody can be used as Rabbit IgG control in FC.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for GST Tag antibody 10000-0-AP | Download protocol |
| WB protocol for GST Tag antibody 10000-0-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Seryl tRNA synthetase cooperates with POT1 to regulate telomere length and cellular senescence. | ||
Signal Transduct Target Ther A dual-role of SARS-CoV-2 nucleocapsid protein in regulating innate immune response. | ||
Signal Transduct Target Ther SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis | ||
Signal Transduct Target Ther Visinin-like protein 1 disrupts calcium homeostasis and promotes atrial fibrillation in human and rodent models | ||
Signal Transduct Target Ther The noncanonical function of liver-type phosphofructokinase potentiates the efficacy of HDAC inhibitors in cancer. | ||
Cancer Cell B cells disrupt tertiary lymphoid structure formation and suppress anti-tumor immunity. |
