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验证数据展示

  • WB analysis using 27872-1-AP

    WB analysis using 27872-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27872-1-AP (Gamma Catenin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis using 27872-1-AP

    WB analysis using 27872-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27872-1-AP (gamma catenin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 27872-1-AP

    IHC staining of human breast cancer using 27872-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27872-1-AP (Gamma Catenin antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with JUP antibody included
  • IHC staining of human breast cancer using 27872-1-AP

    IHC staining of human breast cancer using 27872-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27872-1-AP (Gamma Catenin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with JUP antibody included
  • IF Staining of HeLa using 27872-1-AP

    IF Staining of HeLa using 27872-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 27872-1-AP (gamma catenin antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of A431 using 27872-1-AP

    IF Staining of A431 using 27872-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Gamma Catenin antibody (27872-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of MCF-7 using 27872-1-AP

    IF Staining of MCF-7 using 27872-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Gamma Catenin antibody (27872-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

Gamma Catenin Polyclonal antibody

Gamma Catenin Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 27872-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA and More (1)

别名

JUP, JUP/gamma Catenin, γ-Catenin, ARVD12, Catenin gamma

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHT-29 cells, HUVEC cells, T-47D cells, mouse heart tissue, MCF-7 cells, rat heart tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells, HeLa cells, A431 cells
Planning an IHC experiment? We recommend our IHCeasy JUP Ready-To-Use IHC Kit. JUP primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27872-1-AP targets Gamma Catenin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原

CatNo: Ag27394

Product name: Recombinant human JUP protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 669-745 aa of BC000441

Sequence: TNSLFKHDPAAWEAAQSMIPINEPYGDDMDATYRPMYSSDVPLDPLEMHMDMDGDYPIDTYSDGLRPPYPTADHMLA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 junction plakoglobin
别名 JUP, JUP/gamma Catenin, γ-Catenin, ARVD12, Catenin gamma
观测分子量 82 kDa
GenBank蛋白编号BC000441
基因名称 Gamma Catenin
Gene ID (NCBI) 3728
RRIDAB_2881000
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP14923
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for Gamma Catenin antibody 27872-1-APDownload protocol
IHC protocol for Gamma Catenin antibody 27872-1-APDownload protocol
WB protocol for Gamma Catenin antibody 27872-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Oncol (Dordr)

The POU2F1/miR-4490/USP22 axis regulates cell proliferation and metastasis in gastric cancer.

Authors - Yizhi Xiao
View Article
humanWB,IF

BMC Cancer

Systematic optimization and evaluation of culture conditions for the construction of circulating tumor cell clusters using breast cancer cell lines

Authors - Jueyao Zou
View Article
humanWB,IF

Int J Biol Macromol

Frameshift variants in the C-terminal of CTNNB1 cause familial exudative vitreoretinopathy by AXIN1-mediated ubiquitin-proteasome degradation condensation

Authors - Yining Liu
View Article
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